The Minute™ Total Protein Extraction Kit for Adipose Tissues/Cultured Adipocytes is the best way to extract proteins from fat tissues. It uses a lysis buffer stronger than RIPA buffer and combines it with a spin column-based filter to produce a complete protein profile, which RIPA does not. https://inventbiotech.com/products/minute-total-protein-extraction-kit-for-adipose-tissues-cultured-adipocytes?_pos=1&_sid=4dc367ec1&_ss=r

Hello, about your question, it is firstly defatted sample by using suitable solvent (according to polarity of sample)

Defatting process;I mean study characters of fat in sample in which isolate protein is available . if it is polar select polar solvent for defatting sample such as ethanol or chloroform. if it is non polar select non polar solvent such as hexane or petroleum ether