Dear all,
Has anyone experience what happens if genomic DNA is spiked to a plasma sample from which DNA is extracted using the QIAamp Circulating Nucleic Acid Extraction Kit? If we add different amounts of genomic DNA, will the yield after extraction be proportional to the input or not, as there is a preferential purification of the small fragments of cfDNA over the high molecular weight genomic DNA? Would a restriction digest of the genomic DNA Prior to spiking solver such a potential problem? Thanks for sharing your experiences!
Hi,
if the sample to be processed is spiked with genomic DNA, the output will definitely depend on the input. However, as these binding columns have a size cutoff a lot of high molecular weight genomic DNA may not go through. As mentioned restriction digestion should help to certain extent.
Hello
We used AluI to digest genomic DNA then spiked to the plasma samples. Please
read this paper:
Article Selective capture of plasma cell-free tumor DNA on magnetic ...
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