DNA extraction from human hair using QIAamp DNA Mini Kit?

Does anone have experience using the QIAamp DNA Mini Kit for the extraction of DNA from human hair? How much of starting material will be necessary. How is the pretreatment of the sample done...

25 October 2018 1,129 1 View

Experiences using Oneservice Blood Mini QC kit compared to QIAamp DNA Blood Mini Kit on Qiacube?

Dear all, has anyone used the Blood Mini QC kit from Oneservices with a Qiacube? If yes, how does this kit perform compared to the Qiagen QIAamp DNA Blood Mini Kit regarding yield and sample...

25 March 2018 6,476 1 View

Are there any established parameters to evaluate signal quality of EMG?

20 April 2016 9,120 5 View

How can I improve reproducibility of the TUNEL assay in plants?

Dear all, is anyone of you experienced how to improve the reproducibility of TUNEL assay in plant/moss cell culture? I am trying to establish this assay to visualize DNA damages in nuclei. When I...

31 May 2015 7,727 3 View

How can I isolate protein from fat tissue?

I am trying to isolate membrane bound protein from a number of different mouse tissues and I wanted to know if high salt (400mM) RIPA buffer would work on fat.

10 June 2014 3,564 3 View

How can I stain primary B cells (mouse) with an antibody against mouse without having all their antibodies detected by the anti mouse sec. ab in IF?

I already tried blocking, which was fine for lines, but the B cells present so many antibodies that I cannot cover. The secondary antibody control still shows massive cytoplasmic/membrane...

20 February 2013 2,989 11 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View