How to fix the voids on a floodplain map by using HEC_RAS model?

24 January 2021 9,054 2 View

What are the first steps to learn to write meta-analysis ???

any suggestions for beginners ???

31 December 2020 3,055 4 View

How can we guide students towards active learning? What are the activities for such practices?

I am asking this in relation to practice in active learning in physics education in context of Nepal's education system where we highly focus on content based and rote learning.

02 December 2020 6,686 3 View

How to create a index using principal component analysis?

Hi All I need your guidance on how we can combine multiple variables and develop one index. The goal is to have a index based of which we can plan for staffing. The variables are: daily patient...

21 October 2020 422 6 View

How to remove abnormal behavior at support of FE model of RCC Beam?

Hi, I am modelling a half model of RCC beam in ANSYS Mechanical APDL. The model I have created is exhibiting abnormal behavior at the support with abnormal cracks. I would be grateful if you...

12 October 2020 8,596 3 View

How are Spatial Cognition and cartography interrelated? What are their major uses and functions in field of cartography?

Major details about their interrelations, their major uses and functions.

22 September 2020 9,796 4 View

How to effectively extract DNA from dairy cattle's milk?

Does anyone have experience of extracting DNA out of dairy cow's milk? I am interested in the DNA present in the B-cells. I have done extraction from blood but want to try extraction from milk. I...

02 September 2020 3,536 5 View

How to calibrate the parameters of coupled damage-plasticity microplane model of concrete for FE modelling of RCC beam in ANSYS Mechanical APDL?

Hi, I am trying to model an RCC beam in ANSYS Mechanical APDL. I have the value of Compressive strength, and modulus of elasticity of the concrete of the experiment I am trying to validate ? Can...

28 August 2020 4,623 2 View

In what concentration should i preserve bovine PBMC in 10% DMSO 90% HS for B-cell count via flow cytometry later on?

I want to preserve bovine PBMCs for doing flow cytometry later on. It doesn't seem feasible to count PBMC cells for preservation as there will be lots of samples. Is resuspending the PBMC pellets...

07 July 2020 8,228 2 View

Is ehtical clearance mandatory for research?

Is ehtical clearance mandatory for research? As far as I understand where you have sensitive design and experiments or issues related to human and animals, there only you are required to go with...

23 May 2020 538 6 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View