Recently I am struggling to purify the full-Length human PARP1 using both pet28a and pet21b vectors. If anyone is getting good yield, protocol please?

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Prem Shrestha

Sebastian Schmitt

Thank you.

Michael J. Benedik

Although it is big, it isn't too big for bacteria (LacZ is larger for example). Check out the following

Purification of DNA Damage-Dependent PARPs from E. coli for Structural and Biochemical Analysis. DOI: 10.1007/978-1-4939-6993-7_27

Michael J. Benedik Thank you.