What is the mol. weight of GFP-11 tag?

Hi, I am trying to find out the mol. weight of GFP 11 because it is tagged to my protein of interest which is 17kDa. GFP 11 has been tagged with my protein of interest while GFP 1-10 is tagged...

09 December 2020 1,863 1 View

How should I store the milk sample in RNAlater?

I want to extract RNA from human breast milk. How should I store the milk sample in RNAlater? And after RNA extraction how can I store the RNA?

01 December 2020 7,183 4 View

Which is the best stripping method for proteins having molecular weight 90kDa and above?

Hi, I would like to know which is the best stripping method for proteins having mol. wt. around 90 kDa or more. I am using human samples. I used 0.5M EDTA stripping buffer by boiling PVDF...

08 September 2020 5,815 2 View

How to derive parametric equations from a given dataset by Gene Programming Method ?

I have a dataset of some dependent and independent variables. How to get a parametric equation from this. In some paper, it is written that they have created equation from Gene programming method....

24 July 2020 6,979 2 View

How to decide the proper number of elements in edge sizing for tetrahedral meshing ?

For hexahedral meshing, it is quite simple to divide one edge in a particular number of division. But for tetrahedral meshing it is not that simple. If we are using a complicated domain for...

29 June 2020 4,137 6 View

What is the best solvent for dexamethasone so that it does not get toxic for the cells?

Hi, I am using C2C12 cells. I need to treat them with dexamethasone. I would like to know which is the best solvent to dissolve dexamethasone so that it is not toxic to the cells? I am using dex...

22 June 2020 4,870 6 View

Is it possible to create an analytical expression from the artificial neural network toolbox in MATLAB?

Is it possible to create an analytical expression from an artificial neural network toolbox in MATLAB? If not, then how can these datasets be used as objective function in optimization? In some...

10 June 2020 5,753 8 View

How to plot the graph of turbulence intensity in ansys cfx for steady state analysis??

I want to plot the graph of turbulence intensity vs height of domain. How to do that in Ansys cfx for steady state analysis?

20 April 2020 1,993 3 View

How to build alignment files for homomeric and heteromeric proteins using Modeller?

I am building protein structures for a list of query proteins via homology modelling using Modeller. In some cases the template is a homomeric protein and in others its a heteromeric protein....

01 April 2020 6,576 3 View

What will be the analytical solution for two-dimensional unsteady-state heat conduction equation for a cylindrical pipe/rod (cylindrical coordinates)?

Is there any article that provides good insight on the procedure for reaching an analytical solution of this problem, especially when heat is applied at the wall of the cylindrical pipe and the...

04 March 2020 9,092 7 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View