Hi,

I am performing an IP-WB using GFP magnetic agarose beads from chromtek. In the protocol, after washing, they mention that if elution performed by boiling beads with 2X-sds sample buffer and collecting the supernatant for loading on the gel. So my question is if I store this eluted supernatant and store it later on SDS-page gel for WB, is it ok? If so, at what temperature should the input, flow through and eluted fractions be stored for later running on SDS-page gel?