Dose anyone know how to minimise cell death and improve viability of cell if they are transfected via NUCLEOFECTION?

01 January 1970 1 2K Report

I am currently involved in project that need to transfect B cell with siRNA via NUCLEOFECTION ,problem is viability varies 80 to 16 percent.dose any one faced same problem ,if yes please let me know how to solve it ...thanks

Ana Dorrego-Rivas

I use it quite regularly and what I do is the following: the day after, when I change the medium, I only remove half of it and put half of fresh one. I think it is good for the cells to keep some released growth factors.

Of course I guess you have already set up a series of parameters (voltage, duration of the pulse, cell density etc).

I hope it's useful!

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Science method

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