How mirna chose its target among 100s of mRNA having seed sequence?

I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...

21 April 2019 281 4 View

Films prepared by combination of Hypromellose E15 and E5 polymer with 20% of plasticizer (PEG 400) w.r.t dry polymer using water results in brittle ?

This question is related to soluble film formulation.

04 September 2018 3,086 1 View

How to meassure primary miRNA trancript using QRT PCR?

I want to know how to design primer for primary miRNA QRT PCR

05 March 2018 4,842 6 View

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only?

How to draw Scatchard plot, using data of fluorescence intensity and concentrations of ligands and protein only. Please help..

22 January 2018 992 4 View

I wanted to see if all the morphs of nematodes are same species or not by molecular sequencing, how to proceed doing it?

20 March 2017 5,885 3 View

What is the function of acetic acid and methanol in staining-destaining of sds page?

function of acetic acid and methanol in staining-destaining of sds page

17 February 2017 3,392 4 View

Can miRNA mimics or inhibitor used in primary cells?

I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells

02 November 2016 1,753 3 View

How to reduce size of a void in a metal from micron to nanometer range ??

I want to know if there is a way in which the void of the material inside the metal can be divided from micron size to nanometer size by splitting the voids into discrete voids of reduced size...

03 October 2016 6,811 3 View

How is the molecular phylogenetic analysis of foraminifera beneficial for taxonomic studies?

I would like to know the actual role of rDNA and protein sequences in evolutionary relationships of foraminifera.

23 August 2016 9,210 2 View

Has anyone implemented Self Organizing (Adaptive) Fuzzy controller?

I have read a lot theory about Self Organizing Fuzzy control from Internet. But I am still not completely able to understand its theory and implementation.I have attached one of the best document...

02 June 2016 2,438 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Markers of mitochondrial activity in FFPE tissues?

Hello everybody, Does someone have any experience with assessing/extimating mitochondrial activity on FFPE tissues? Precisely, no TOM20, ATP5B, etc: we are trying to get an approximative idea of...

24 February 2021 4,618 4 View