Satyajeet Salunkhe

15 Questions 26 Answers 0 Followers

Questions related from Satyajeet Salunkhe

How mirna chose its target among 100s of mRNA having seed sequence?

I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...

22 April 2019 351 4 View

How to meassure primary miRNA trancript using QRT PCR?

I want to know how to design primer for primary miRNA QRT PCR

06 March 2018 4,923 6 View

How will I design RNA ip then qrt pcr experiment?

I want to perform RNA ip ,and by qrt pcr I want to analyse the RNA,but I am not sure how ,I will set up experiment, How I will normalize the RNA oulled down to input, RNA Ip will give one Ct and...

24 January 2017 164 1 View

Can miRNA mimics or inhibitor used in primary cells?

I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells

03 November 2016 1,842 3 View

Sequence of my gene of interest exactly matching to one pseudogene can it interfere in Real time based assay?

Gene of my interest has exactly similarity to pseudogene.Can i still design the real time primer in same region,my question is do pseudogene will have impact on the detection original transcript...

29 June 2016 4,701 1 View

How will Self renewal property of B lymphocyte can be assyed experimentally?

I am working on B cell differentiation ,I want to know how self renewal can be experimentally assayed in these cells.

14 January 2016 6,832 2 View

Can anyone help me with the interpretation of intron centric analysis file output ?

RNA seq data analysis by intron centric analysis gives insight about exon skkiping ,I have attached photo of excel file I have. Can anyone help me how to interpretate it eg...

05 July 2015 2,094 3 View

Can anybody help me to develop a model for cellular differentiation?

Cellular transcription factors are critical for differentiation of particular cell, but there may be some critical transcription factor playing a vital role in differentiation, so can mathematical...

03 February 2015 7,943 1 View

How can one validate co-immunoprecipitation experiments?

If one wants to see the interaction between two proteins by immunoprecipitation. How can one validate the co-immunoprecipitation experiment?

13 October 2014 7,063 10 View

How can I predict DNA binding site in my protein?

I have protein that has DNA binding activity. I want to know how I can predict which part of my protein is interacting with DNA by in silico method.

18 June 2014 9,684 2 View

Can I use a normal genomic DNA isolation kit for isolation of apoptotic DNA of cancer cell?

We have MN kit in our lab nucleo spin, but I want to know whether it can be used for isolation of apoptotic DNA.

16 June 2014 937 3 View

What are the limitations of a MTT assay in a cell viability study?

In my experiment I got false MTT activity in-spite drug was supposed to be cytotoxic I just want to get insight about limitations of MTT.

09 June 2014 6,674 9 View

What is the role of 300 mM NaCl used to prepare buffers used for protein samples?

I just want to know why the buffer contains the NaCl.

06 May 2014 6,502 8 View

Can any one provide protocol for MIRVANA mirna mimic /inhibitor nucleofection by NUCLOEFECTOR 4D X?

I want to transfect mirna mimic /inhibitor to primary B cells,Can any one provide the protocol for Nucleofection of mirvana mimic ,inhibitor via nucleofector4 D X

01 January 1970 1,093 1 View

Dose anyone know how to minimise cell death and improve viability of cell if they are transfected via NUCLEOFECTION?

I am currently involved in project that need to transfect B cell with siRNA via NUCLEOFECTION ,problem is viability varies 80 to 16 percent.dose any one faced same problem ,if yes please let me...

01 January 1970 2,460 1 View