22 Questions 35 Answers 0 Followers
Questions related from Satyajeet Salunkhe
Hi, For compensation, we can use single-stained beads in flow cytometry. For gating control or FMO: can we just mix single-stained beads to get FMO?
26 May 2024 6,793 1 View
In addgene 84323, plasmid they have empty gRNA casette. I am not sure where to clin guide RNA of interest. 1. Is it should be between U6 promoter and gRNA casette or 2. In gRNA casette itself?
05 April 2024 8,119 3 View
In 84232 add gene plasmid, I am not sure at which site should I clone guide RNA
05 April 2024 7,984 3 View
I am not clear what is rule behind mirna and mRNA targeting pair,because each mirna target officially 100s of target. In such cases how one will validate legitimate mirna mRNA target. Assays...
22 April 2019 371 4 View
I want to know how to design primer for primary miRNA QRT PCR
06 March 2018 4,943 6 View
Excel plots Yon x ,regression line,so in practical situation in preparation of standard curve of known concentration of proteain to OD , One have to plot concentration on y axis and od on x axis...
13 May 2017 3,520 6 View
I want to perform RNA ip ,and by qrt pcr I want to analyse the RNA,but I am not sure how ,I will set up experiment, How I will normalize the RNA oulled down to input, RNA Ip will give one Ct and...
24 January 2017 173 1 View
I am currently working with lymphocyte ,I want to know if any one has used the miRNA mimics or inhibitor with primery cells
03 November 2016 1,861 3 View
i want to analyse region on dna of gene of known transcrition start site ,what should be region i will take upstream and downstream of tss for to get transcription binding site.
01 October 2016 2,284 4 View
Gene of my interest has exactly similarity to pseudogene.Can i still design the real time primer in same region,my question is do pseudogene will have impact on the detection original transcript...
29 June 2016 4,707 1 View
Currently I designed primer by Primer BLAST but even it showed the no non specific band the real time results showed non specific binding I am not aware of fact whether BLAST take consideration of...
22 January 2016 1,378 1 View
I am working on B cell differentiation ,I want to know how self renewal can be experimentally assayed in these cells.
14 January 2016 6,844 2 View
RNA seq data analysis by intron centric analysis gives insight about exon skkiping ,I have attached photo of excel file I have. Can anyone help me how to interpretate it eg...
05 July 2015 2,107 3 View
Cellular transcription factors are critical for differentiation of particular cell, but there may be some critical transcription factor playing a vital role in differentiation, so can mathematical...
03 February 2015 7,954 1 View
If one wants to see the interaction between two proteins by immunoprecipitation. How can one validate the co-immunoprecipitation experiment?
13 October 2014 7,071 10 View
during discussion with relevence to enzyme active site we come to know that amino acids at active site have different pka as compared to when these amino acids present in free form if anybody had...
30 August 2014 8,753 8 View
I have protein that has DNA binding activity. I want to know how I can predict which part of my protein is interacting with DNA by in silico method.
18 June 2014 9,696 2 View
We have MN kit in our lab nucleo spin, but I want to know whether it can be used for isolation of apoptotic DNA.
16 June 2014 943 3 View
In my experiment I got false MTT activity in-spite drug was supposed to be cytotoxic I just want to get insight about limitations of MTT.
09 June 2014 6,683 9 View
I just want to know why the buffer contains the NaCl.
06 May 2014 6,510 8 View
I want to transfect mirna mimic /inhibitor to primary B cells,Can any one provide the protocol for Nucleofection of mirvana mimic ,inhibitor via nucleofector4 D X
01 January 1970 1,102 1 View
I am currently involved in project that need to transfect B cell with siRNA via NUCLEOFECTION ,problem is viability varies 80 to 16 percent.dose any one faced same problem ,if yes please let me...
01 January 1970 2,470 1 View
