I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as no 1) and another time I worked in a late passage (such as no.4). Does the number of passages show a type of resistant or not to the cytotoxic drugs? The shape and the identity of a cell still conserved. How many passages after it should I withdraw the cell? The cell line is MDA-MB-231.
SURELY, always better to use the cells of initial passages. You should carefully see the size and shape of your cell, any alteration you should reject it.
It depends, carefully see any microplasma contamination is there or not?
I agree with Deepshikha and while washing cells before treatment need to take care. If washing is harsh cells will come off.
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