Which (neuron) cell lines are better for studies about MS disease in vitro?

Which (neuron) cell lines are better for studies about MS disease in vitro?

05 June 2016 3,238 6 View

How can I use small amount of ligand (ng or nanoliter) for experiment?

nano

10 November 2015 111 0 View

I want to use vitamin D (50000 IU) equal to 1.25 mg for my cell culture ,...?

how much ethanol is needed to prepare stock of vitamin D with the concentration of 1 mg/ml , should I use 1 ml ethanol or more?

10 November 2015 6,528 3 View

In my MTT test, when I use higher concentration of drug for treatment, there is higher absorbance than in the control. Can anyone help me?

In my MTT test, when I use higher concentration of drug for treatment, there is higher absorbance than in the control. Can anyone help me?

08 September 2015 3,309 5 View

Does anyone know that aminolevulinic acid (ALA) has absorbance in water solution (in vitro) by UV/Vis spectroscopy or not? why?

aminolevulinic acid (ALA), PDT, Drug

05 June 2015 6,780 4 View

Can we dye cells with alizarin red S or oil red with out fixing cells?

dye cells with alizarin red S or oil red( for differentiation test)

01 February 2015 4,810 1 View

Is there a dilution factor for trypan blue exclusion assay, when we use 90 micromolar cell sample and 10 micromolar trypan blue?

Trypan blue exclusion assay dilution factor

11 December 2014 7,104 2 View

Does the number of cell passages affect the activity of drug or metal?

I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as no...

11 December 2014 8,598 5 View

What is the best method for detecting singlet oxygen in cell culture studies?

09 October 2014 9,646 1 View

What breast cancer cell lines are better for cellular experiment particulary for apoptosis study?

MDA-231 MCF-7 OR....

09 October 2014 8,421 5 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View