Which (neuron) cell lines are better for studies about MS disease in vitro?

Which (neuron) cell lines are better for studies about MS disease in vitro?

05 June 2016 3,238 6 View

Which (neuron) cell lines are better for studies about Alzheimer's disease as model in vitro?

Alzheimer's disease neuronal cell line

05 June 2016 2,594 4 View

I want to use vitamin D (50000 IU) equal to 1.25 mg for my cell culture ,...?

how much ethanol is needed to prepare stock of vitamin D with the concentration of 1 mg/ml , should I use 1 ml ethanol or more?

10 November 2015 6,528 3 View

In my MTT test, when I use higher concentration of drug for treatment, there is higher absorbance than in the control. Can anyone help me?

In my MTT test, when I use higher concentration of drug for treatment, there is higher absorbance than in the control. Can anyone help me?

08 September 2015 3,309 5 View

Does anyone know that aminolevulinic acid (ALA) has absorbance in water solution (in vitro) by UV/Vis spectroscopy or not? why?

aminolevulinic acid (ALA), PDT, Drug

05 June 2015 6,780 4 View

Can we dye cells with alizarin red S or oil red with out fixing cells?

dye cells with alizarin red S or oil red( for differentiation test)

01 February 2015 4,810 1 View

Is there a dilution factor for trypan blue exclusion assay, when we use 90 micromolar cell sample and 10 micromolar trypan blue?

Trypan blue exclusion assay dilution factor

11 December 2014 7,104 2 View

Does the number of cell passages affect the activity of drug or metal?

I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as no...

11 December 2014 8,598 5 View

What is the best method for detecting singlet oxygen in cell culture studies?

09 October 2014 9,646 1 View

What breast cancer cell lines are better for cellular experiment particulary for apoptosis study?

MDA-231 MCF-7 OR....

09 October 2014 8,421 5 View

What is Nano technology with coronavirus helpe the human..and is it nano mterals removal of covid19?

I anwar by adsorption or reaction ..but it is either more method...

02 March 2021 4,811 2 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Zno nano particles colour changed from white to grey in process of calcination any suggestions?

In the formation of Zno nano particles process, after drying at 120 degree C, my zno nano particles are completely white but when i calcine it for 450 degree C it converts into grey colour. i...

26 February 2021 2,435 4 View

QPCR tubes for Roche Lightcycler Nano?

We own a small cPCR thermal cycler from Roche, Lightcycler Nano, which is convenient for backup quick runs. Recently, Roche has discontinued the plastics for it and they offer no alternative from...

17 February 2021 8,694 5 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Best synthesis method for large scale silver nanoparticle synthesis with high yield?.Best post processing steps after nanoparticle synthesis?

I have chemically synthesized silver nanoparticle to combat microbes on wound site. I have used many methods of synthesis, but I found minimal field of silver NP product.I am getting whitish...

11 February 2021 4,964 13 View

How to check if the PMF profile obtained from ABF simulation is correct?

I am doing ABF simulations to calculate ligand binding enegy. The PMF profile that I get using single long ABF simulation is attached as pmf_total.png. When I do this ABF calculation with 10...

10 February 2021 4,170 2 View

Dear colleagues. How can we implement the kinetics of the polymerization of aromatic amines in easier and simpler ways?

Dear colleagues. How can we implement the kinetics of the polymerization of aromatic amines in easier and simpler ways? Are there simpler methods for Nano polymers (other than those obtained with...

09 February 2021 5,783 3 View