nano
Which (neuron) cell lines are better for studies about MS disease in vitro?
05 June 2016 3,303 6 View
Alzheimer's disease neuronal cell line
05 June 2016 2,658 4 View
how much ethanol is needed to prepare stock of vitamin D with the concentration of 1 mg/ml , should I use 1 ml ethanol or more?
10 November 2015 6,595 3 View
In my MTT test, when I use higher concentration of drug for treatment, there is higher absorbance than in the control. Can anyone help me?
08 September 2015 3,377 5 View
aminolevulinic acid (ALA), PDT, Drug
05 June 2015 6,884 4 View
dye cells with alizarin red S or oil red( for differentiation test)
01 February 2015 4,884 1 View
Trypan blue exclusion assay dilution factor
11 December 2014 7,175 2 View
I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as no...
11 December 2014 8,694 5 View
MDA-231 MCF-7 OR....
09 October 2014 8,477 5 View
Do you have any suggestion on assays for detecting singlet oxygen in cell culture?
09 October 2014 9,712 1 View
Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...
05 August 2024 8,946 3 View
why don't d-orbitals split themselves because of themselves without the presence of ligands? Electrons are indistinguishable. Why wouldn't it be more correct that protons from a ligand split the...
03 August 2024 3,589 3 View
I'm guessing it's because the ligand experiences too much electron repulsion or proton repulsion from the chromium to insert them close to the 3d-orbitals which are close to the metal nucleus. Is...
03 August 2024 1,370 1 View
Hello,, The metal complex ligand appears incomplete in the screen of the discovery studio visualizer
30 July 2024 3,354 1 View
Good day, I am a student trying to work on Autodock for a project regarding Ligand-DNA interaction so i am quite new to molecular docking. i have followed tutorials and did all the steps...
28 July 2024 2,136 4 View
I am performing fluorescence experiments using a ligand to detect metal ions. I want to determine the Lowest Detection Limit (LOD) using the formula LOD = 3σ / K. However, I'm uncertain about...
19 July 2024 1,086 1 View
I have been able to assign the CHARMM forcefield to my ligand i.e. acetyl coenzyme A but not the amber forcefield. I want to run amber forcefield and the parameterization of ligand is failing. I...
15 July 2024 7,323 1 View
Hi! I am currently working on a protein where I have selected residues within 10A of its bound ligand in pymol. However, I can not find a way to print out what residues are contained in that group...
15 July 2024 1,727 1 View
Hello! everyone, I am trying to study in silico Protein-Protein and Protein-RNA interactions. Now, is there any tool with which I can identify the interacting amino acid residues or the...
14 July 2024 950 2 View
During the preparation of a ligand molecule in autodock or openbabel Cl atom is not showing in the .pdbqt file whereas it was present in the pdb and sdf file. Due to this Cl atom is not showing in...
13 July 2024 3,486 6 View
