Which (neuron) cell lines are better for studies about MS disease in vitro?
Hi Khatereh,
You may want to look at oligodendroglial cell lines even though it is far from being the "right" model. Another aspect to consider is that MS is a complex disorder with different subtypes that likely do not share the same underlying mechanisms. As far as in vitro studies are concerned, human embryonic stem cells containing MS genomes might be a way to go, but arguably more challenging than a simple cell line.
Hope this helps. Norbert
Hi,
Thanks for your answer, you are right , MS is complex disorder but I wanted to study molecular and cellular mechanisms ( mainly biochemical) underlying MS disease via cell studies. I don't know is it possible or not?/
I think that oligodendrocyte cell lines are usually used to study MS disease. I found 2 articles related to MS models.
I think you need to think about what aspect of MS that you want to recapitulate in vitro. That is challenging since there is the autoimmune component. I imagine you could utilize a co-culture system either DRG neurons + oligodendrocytes or perhaps a slice preparation. How you would trigger an autoimmune response is less clear. Do you want to specifically look at remyelination mechanisms or demyelination. It would be easier to utilize an oligodendrocyte cell line rather than harvest OPCs.
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