Which (neuron) cell lines are better for studies about MS disease in vitro?

Which (neuron) cell lines are better for studies about MS disease in vitro?

05 June 2016 3,238 6 View

Which (neuron) cell lines are better for studies about Alzheimer's disease as model in vitro?

Alzheimer's disease neuronal cell line

05 June 2016 2,594 4 View

How can I use small amount of ligand (ng or nanoliter) for experiment?

nano

10 November 2015 111 0 View

I want to use vitamin D (50000 IU) equal to 1.25 mg for my cell culture ,...?

how much ethanol is needed to prepare stock of vitamin D with the concentration of 1 mg/ml , should I use 1 ml ethanol or more?

10 November 2015 6,528 3 View

In my MTT test, when I use higher concentration of drug for treatment, there is higher absorbance than in the control. Can anyone help me?

In my MTT test, when I use higher concentration of drug for treatment, there is higher absorbance than in the control. Can anyone help me?

08 September 2015 3,309 5 View

Can we dye cells with alizarin red S or oil red with out fixing cells?

dye cells with alizarin red S or oil red( for differentiation test)

01 February 2015 4,810 1 View

Is there a dilution factor for trypan blue exclusion assay, when we use 90 micromolar cell sample and 10 micromolar trypan blue?

Trypan blue exclusion assay dilution factor

11 December 2014 7,104 2 View

Does the number of cell passages affect the activity of drug or metal?

I am doing a cytotoxicity study for a group of drugs in different cell lines using an MTT assay. I am wondering about cell passage (Subculturing). One time I worked in an early passage (such as no...

11 December 2014 8,598 5 View

What is the best method for detecting singlet oxygen in cell culture studies?

09 October 2014 9,646 1 View

What breast cancer cell lines are better for cellular experiment particulary for apoptosis study?

MDA-231 MCF-7 OR....

09 October 2014 8,421 5 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

How can I simulate the optical absorption spectrum of a biomaterial (like DNA or protein) in COMSOL?

I need to simulate the optical properties of a biomaterial, namely DNA, in COMSOL. Basically I need to simulate the IR absorption spectrum of a cylinder filled with a 'concentration' of DNA. I am...

21 February 2021 3,043 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Unusually Busy Raman Background?

Hello, I am observing a nearly periodic background spectrum for a wide variety of SERS substrates. I see this response (attached below) for bare Au films, Cu films, and Au nanoparticles on...

15 February 2021 9,922 5 View

How to determine the drug loading and the drug release capacity of a polymer micelle using UV spectroscopy?

Currently, I am working on PLA-PEG copolymer micelle for the drug delivery system. I have seen several papers where some researcher talks to centrifuge the drug-loaded micelle to separate the...

13 February 2021 5,822 7 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View