Trypan blue exclusion assay
dilution factor
Hi Khatereh, please take a look at this protocol:
http://www.lifetechnologies.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/trypan-blue-exclusion.html
From a 0.4% solution we use 1:1 with cell medium (10 uL of each and 10 uL of that solution) onto a Countess automated counter slide. For manual counting 1:10 is recommended I believe.
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