Hi everyone,
I wanna use Cell titer Glo for my spheroids, my spheroids are in the diameter between 350- 390 micrometer, in the protocol it is written the 100 micro liter is enough for every spheroid, but I have heard it is possible to use less amount of it, if someone has information and experience using it previously I would appreciate it if tell me how much is enough for this size of spheroid?
also if it is needed using negative control and positive control, I would appreciate explaining how should i prepare them?
Hey there, have you tried contacting the tech_serv at your local Promega branch? They are usually pretty fast in replying and they have application centers that have tested their kits for different applications. So reach out to them and see what they say. And then, if you have spheroids that you can run a trial on, you could try different volumes and see which is the minimum you need for your experiments. Everybody's set-up is different so what works for someone, may not work for you.
Klavdja A. Fignole thanks alot for your suggestion, I sent my question but still I did not get any reply. if you did contact successfully can I ask share it.
Hey Elmira! Try sending it to [email protected]. The techserv in Switzerland, typically replies within 24h or less. If the German one doesn't answer you, let me know and I'll ask the ones in Zurich.
I am not sure about my answer and so I think it's best to check with them. I don't want to have you waste reagents and time.
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