Can somebody help me with a contamination in primary retinal pigment epithelium cells culture?

Hi friends, In the attached picture, you can see the primary RPE cells contaminated with small motile rode shape bacteria in 40 magnification microscope image. i cultured the extracted cells in...

11 December 2019 8,270 3 View

Can somebody explane the depth z-slice images of cellular spheroids ?

Hi everybody, I prepared some z-stack images from spheroid, and the z-slice in depth shows a in a same diamater of spheroid a round circle , As i understood from definition of z-stack image it...

11 December 2019 3,266 2 View

Is there any possibility to obserev an specific protein in a autoflourescence microtissue?

Hi every body, I prepared a microtisssue from autoflourescence cells, which when I did control negative flourescence staining for that (without using primary antibody) and using a redemitting...

11 December 2019 4,526 2 View

Does anybody cultured primary RPE cells in higher antibiotich concentration of 2%?

I did primary RPE cells extraction from the fresh pig eyes, but after 3 days of seeding cells the medium (miller medium including 10% pig serum and 2% penicillul/streptomycin) is looking cloudy(...

10 November 2019 1,480 3 View

Can anybody explain the effect of the plate culture on the differentiation of the RPE cells?

Hi every body, I have read in some papers that if RPE cells are seeded in flask very densly, they would differentiate in other cells? But I see their differentioation when they were made some...

09 October 2019 3,146 1 View

Did anybody do TUNEL assay to visualize the apoptotic nulei in cryosectioned/parafinnized spheroids slides?

Hi colleagues, I have to do tunel assayy to observe the nuclei structure of spheroids. But the problem is that cryosection in frozen spheroids in tissue tec make a very sensitive slide sample...

08 September 2019 6,159 3 View

Does the kit for Cell titer Glo( spheroids) needs control positive and negative ?

Hi everyone, I wanna use Cell titer Glo for my spheroids, my spheroids are in the diameter between 350- 390 micrometer, in the protocol it is written the 100 micro liter is enough for every...

08 September 2019 8,815 5 View

Can anyone suggest me a simple protocol for prepairing spheroids from normal cells using methyl cellulose?

Hi everyone, I have tried to generate spheroids from primary normal RPEcells using methylcellulose in 96 well plates _U bottom based on some papers but it doesnot work. if some one has...

06 July 2019 2,408 1 View

Has anyone used hanging drop method for harvesting spheroid from primary RPE cells?

Hi, As harvesting spheroid from primary cells takes longer than cell line and as hanging drop method is best method among others is best for spheroid formation, the changing medium would be an...

06 July 2019 1,896 2 View

Conversion in MTF(modulation transfer function) graph?

How can we convert a MTF graph with lines/mm to a graph with cycle/deg unit?

01 February 2019 4,940 1 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

How to calculate Total Flavonoid?

I analyzed flavonoid total spectrophotometry, the procedure as below: 2 ml supernatan added 5 ml dd.H2O, then added 0,15 ml NaNO2 5%, 5 minutes later added 0,15 ml AlCl3 10%, After 6 minutes,...

01 March 2021 6,722 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anyone that can help with details in phosphopeptides enrichment?

Anyone know the amount of protein before enzymatic digestion for phosphopeptides enrichment of serume sample in Ti-IMAC? Has anyone ever use Ti-IMAC(the material is from Chinese company named...

28 February 2021 7,310 3 View