Hi friends,
In the attached picture, you can see the primary RPE cells contaminated with small motile rode shape bacteria in 40 magnification microscope image. i cultured the extracted cells in Miller medium included 2%pen-strep and 10%pig serum. the bacterial contamination can be removed(not always) if i change medium every day . If not, under microscope i can see these bacteria which are motile and are moving in the living cells and in the space between them and after some day the cells all will be dead .
I think they are not mycoplasma. because I see them individually under 40 magnification microscope, how is your opinion?
can some body help me
1)which antibiotics can remove them?
2)what kind of microbes are there?
Hi Elmira!
I think it is a bacillus bacteria and if it is true you can use Tetracycline, chloramphenicol, clindamycin, and erythromycin.
It is bacterial contamination. If you want to check it, inoculate a loopful of this contaminated medium on Nutrient agar plate and look for the bacterial colonies that appear on it after 1-2 days incubation at 37 C. You can perform Gram staining to see whether the contamination is of Gram negative or positive bacteria. however, you may use Gentamicin/ Geneticin at 50 microgram/mL concentration in the tissue culture medium along with PenStrep. The injection vial [Usually 2 mL] having 40 mg/mL concentration may be procured and used in the tissue culture medium. It shall work.
I used bacaway from applied biological materials, it has save my cells many times
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