Can someone suggest a sterilized, clean and trustable pig serum company for ordering?

Hi everyone, I currently did some experiments with a pig serum (notnheat inactivated), which the control negative of that showed bacterial contamination. As I have to filter it befor using and I...

11 December 2019 9,794 3 View

Can somebody help me with a contamination in primary retinal pigment epithelium cells culture?

Hi friends, In the attached picture, you can see the primary RPE cells contaminated with small motile rode shape bacteria in 40 magnification microscope image. i cultured the extracted cells in...

11 December 2019 8,270 3 View

Can somebody explane the depth z-slice images of cellular spheroids ?

Hi everybody, I prepared some z-stack images from spheroid, and the z-slice in depth shows a in a same diamater of spheroid a round circle , As i understood from definition of z-stack image it...

11 December 2019 3,266 2 View

Is there any possibility to obserev an specific protein in a autoflourescence microtissue?

Hi every body, I prepared a microtisssue from autoflourescence cells, which when I did control negative flourescence staining for that (without using primary antibody) and using a redemitting...

11 December 2019 4,526 2 View

Does anybody cultured primary RPE cells in higher antibiotich concentration of 2%?

I did primary RPE cells extraction from the fresh pig eyes, but after 3 days of seeding cells the medium (miller medium including 10% pig serum and 2% penicillul/streptomycin) is looking cloudy(...

10 November 2019 1,480 3 View

Did anybody do TUNEL assay to visualize the apoptotic nulei in cryosectioned/parafinnized spheroids slides?

Hi colleagues, I have to do tunel assayy to observe the nuclei structure of spheroids. But the problem is that cryosection in frozen spheroids in tissue tec make a very sensitive slide sample...

08 September 2019 6,159 3 View

Does the kit for Cell titer Glo( spheroids) needs control positive and negative ?

Hi everyone, I wanna use Cell titer Glo for my spheroids, my spheroids are in the diameter between 350- 390 micrometer, in the protocol it is written the 100 micro liter is enough for every...

08 September 2019 8,815 5 View

Can anyone suggest me a simple protocol for prepairing spheroids from normal cells using methyl cellulose?

Hi everyone, I have tried to generate spheroids from primary normal RPEcells using methylcellulose in 96 well plates _U bottom based on some papers but it doesnot work. if some one has...

06 July 2019 2,408 1 View

Has anyone used hanging drop method for harvesting spheroid from primary RPE cells?

Hi, As harvesting spheroid from primary cells takes longer than cell line and as hanging drop method is best method among others is best for spheroid formation, the changing medium would be an...

06 July 2019 1,896 2 View

Conversion in MTF(modulation transfer function) graph?

How can we convert a MTF graph with lines/mm to a graph with cycle/deg unit?

01 February 2019 4,940 1 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View