Elmira Keramatfar

11 Questions 9 Answers 0 Followers

Questions related from Elmira Keramatfar

Can someone suggest a sterilized, clean and trustable pig serum company for ordering?

Hi everyone, I currently did some experiments with a pig serum (notnheat inactivated), which the control negative of that showed bacterial contamination. As I have to filter it befor using and I...

12 December 2019 9,891 3 View

Can somebody help me with a contamination in primary retinal pigment epithelium cells culture?

Hi friends, In the attached picture, you can see the primary RPE cells contaminated with small motile rode shape bacteria in 40 magnification microscope image. i cultured the extracted cells in...

12 December 2019 8,363 3 View

Is there any possibility to obserev an specific protein in a autoflourescence microtissue?

Hi every body, I prepared a microtisssue from autoflourescence cells, which when I did control negative flourescence staining for that (without using primary antibody) and using a redemitting...

12 December 2019 4,601 2 View

Can somebody explane the depth z-slice images of cellular spheroids ?

Hi everybody, I prepared some z-stack images from spheroid, and the z-slice in depth shows a in a same diamater of spheroid a round circle , As i understood from definition of z-stack image it...

12 December 2019 3,343 2 View

Does anybody cultured primary RPE cells in higher antibiotich concentration of 2%?

I did primary RPE cells extraction from the fresh pig eyes, but after 3 days of seeding cells the medium (miller medium including 10% pig serum and 2% penicillul/streptomycin) is looking cloudy(...

11 November 2019 1,571 3 View

Can anybody explain the effect of the plate culture on the differentiation of the RPE cells?

Hi every body, I have read in some papers that if RPE cells are seeded in flask very densly, they would differentiate in other cells? But I see their differentioation when they were made some...

10 October 2019 3,247 1 View

Did anybody do TUNEL assay to visualize the apoptotic nulei in cryosectioned/parafinnized spheroids slides?

Hi colleagues, I have to do tunel assayy to observe the nuclei structure of spheroids. But the problem is that cryosection in frozen spheroids in tissue tec make a very sensitive slide sample...

09 September 2019 6,242 3 View

Does the kit for Cell titer Glo( spheroids) needs control positive and negative ?

Hi everyone, I wanna use Cell titer Glo for my spheroids, my spheroids are in the diameter between 350- 390 micrometer, in the protocol it is written the 100 micro liter is enough for every...

09 September 2019 8,905 5 View

Can anyone suggest me a simple protocol for prepairing spheroids from normal cells using methyl cellulose?

Hi everyone, I have tried to generate spheroids from primary normal RPEcells using methylcellulose in 96 well plates _U bottom based on some papers but it doesnot work. if some one has...

07 July 2019 2,481 1 View

Has anyone used hanging drop method for harvesting spheroid from primary RPE cells?

Hi, As harvesting spheroid from primary cells takes longer than cell line and as hanging drop method is best method among others is best for spheroid formation, the changing medium would be an...

07 July 2019 1,977 2 View

Conversion in MTF(modulation transfer function) graph?

How can we convert a MTF graph with lines/mm to a graph with cycle/deg unit?

02 February 2019 5,010 1 View