Hi colleagues,
I have to do tunel assayy to observe the nuclei structure of spheroids. But the problem is that cryosection in frozen spheroids in tissue tec make a very sensitive slide sample which just with once washing step the sample completely be removed from slide, I wanna to know weather is it possible to do TUNEl assay for paeraffin embeded spheroid samples ? because this sections seems more stable than in tissue tec frozen spheroid samples.
You can have a look at these references:
1) The Journal of Histochemistry & Cytochemistry, 2000, 48(4), 509
2) PLoS One. 2017; 12(7): e0181340
On Paraffin embedded slides, your tissue will be more stable,
I have done apoptag assay (The ApopTag Peroxidase In Situ Apoptosis Detection Kit detects apoptotic cells in situ by labeling & detecting DNA strand breaks by the TUNEL method) on paraffin sections, has worked very well.
Tunnel assay should work in your case too on paraffin sections.
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