How could i use image j to count specific cells which are labeled by two FITC ab?

how should i adjust image j to count these cells automatically not manually? i put one of my slide image in attached file.

25 December 2020 9,598 2 View

What tests should I run for this small scale research?

25 October 2020 5,690 3 View

Why can't I see band in gel agarose while 260/280 ratio is 1.8 in Nanodrop?

Hi everyone I am trying to extract DNA from soil, 260/280 ratio is 1.8 in nanodrop, concentration is 587 ng/ul. but I can not see the band in gel agarose. Thank you in advance

18 October 2020 9,438 7 View

Error while installing gromacs?

Hello all I was trying to install gromacs 2019 in an non-standard. but i countered this error: /usr/lib/gcc/x86_64-linux-gnu/5/include/mwaitxintrin.h(36): error: identifier...

16 June 2020 3,908 5 View

Number of Antennas for Massive MIMO (Commercially+ acc. to 5G Standardization)?

Currently, most of the reports show that the no. of Tx/Rx antennas for mMIMO (commercially deployed and tested) are upto128 (64T64R-TDD etc). Does anyone knows: 1. What is the number of Tx/Rx...

19 March 2020 3,987 8 View

Could you help me with simulating drag force and dielectrophoresis force in comsol by using AC/DC module?

I'm trying to apply a nonuniform electric field and create a dielectrophoretic force in a channel and put a particle in the middle of the channel. I added these 2 physics, creeping flow and...

13 March 2020 9,721 3 View

What is a good research topic for MA thesis in education or teaching English?

Im thinking to do my thesis in an international level , kind of cross cultural topic. how can I find a grant from a foreign university ?

30 January 2020 5,548 3 View

How do I generate topology of many ligands which are not coonected all of them ,for gromacs?

Hi. I want to do simulate via gromacs for ligand- protein complex which there is 9 ligands (three of them are seperated and six of them are connected together(picture in Add files). when I used...

14 January 2020 5,707 5 View

Can anyone advice how to troubleshoot segmentation fault (core dumped) during NVT equilibration stage of ligand- protein simulation?

Hi. I am using gromose 54a7 force-field and Gromacs package to simulate a protein-ligand complex.it writes pdbs for steps. Maximum force = 8.3529562e+04 But in the NVT equilibration step, the...

16 December 2019 8,409 2 View

How to find nucleotide sequence of a methylation marker ?

I am planing to conduct a research on utilization of methylation pattern to find biological fluids for applications in forensic science. I have selected methylation markers ( For example...

24 November 2019 1,291 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

In frequency domain alpha band(8-16hz), beta band(16-32hz), gamma band(32-64hz), sub-band(64-128hz) are same as LL,LH,HL,HH in wavelet decomposition?

I am working on single trial motor imagery data i.e. EEG data. I am following a paper which has a dataset from BCI challenge IV (dataset 2b). After removing noise and extracting all the data from...

23 February 2021 5,503 1 View