How could i use image j to count specific cells which are labeled by two FITC ab?

how should i adjust image j to count these cells automatically not manually? i put one of my slide image in attached file.

25 December 2020 9,598 2 View

What tests should I run for this small scale research?

25 October 2020 5,690 3 View

Why can't I see band in gel agarose while 260/280 ratio is 1.8 in Nanodrop?

Hi everyone I am trying to extract DNA from soil, 260/280 ratio is 1.8 in nanodrop, concentration is 587 ng/ul. but I can not see the band in gel agarose. Thank you in advance

18 October 2020 9,438 7 View

Does the alpha-synuclein overexpression in SH SY5Y cells have any toxic effect?

Cells are dying post-transfection. I've transfected SH SY5Y cells to overexpress alpha-synuclein, cells were overexpressed but they were dying rapidly. I've checked the overexpression, it was fine...

16 August 2020 7,518 3 View

Error while installing gromacs?

Hello all I was trying to install gromacs 2019 in an non-standard. but i countered this error: /usr/lib/gcc/x86_64-linux-gnu/5/include/mwaitxintrin.h(36): error: identifier...

16 June 2020 3,908 5 View

Number of Antennas for Massive MIMO (Commercially+ acc. to 5G Standardization)?

Currently, most of the reports show that the no. of Tx/Rx antennas for mMIMO (commercially deployed and tested) are upto128 (64T64R-TDD etc). Does anyone knows: 1. What is the number of Tx/Rx...

19 March 2020 3,987 8 View

Could you help me with simulating drag force and dielectrophoresis force in comsol by using AC/DC module?

I'm trying to apply a nonuniform electric field and create a dielectrophoretic force in a channel and put a particle in the middle of the channel. I added these 2 physics, creeping flow and...

13 March 2020 9,721 3 View

What is a good research topic for MA thesis in education or teaching English?

Im thinking to do my thesis in an international level , kind of cross cultural topic. how can I find a grant from a foreign university ?

30 January 2020 5,548 3 View

How do I generate topology of many ligands which are not coonected all of them ,for gromacs?

Hi. I want to do simulate via gromacs for ligand- protein complex which there is 9 ligands (three of them are seperated and six of them are connected together(picture in Add files). when I used...

14 January 2020 5,707 5 View

Can anyone advice how to troubleshoot segmentation fault (core dumped) during NVT equilibration stage of ligand- protein simulation?

Hi. I am using gromose 54a7 force-field and Gromacs package to simulate a protein-ligand complex.it writes pdbs for steps. Maximum force = 8.3529562e+04 But in the NVT equilibration step, the...

16 December 2019 8,409 2 View

Could you recommend any books about Number Theory?

Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.

03 March 2021 7,174 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Samples to study circular dichroism (chirality)?

I want to build a low-cost polarimeter to study circular dichroism (chirality). What type of samples can I use? I am looking for samples that can be found outside the laboratory.

01 March 2021 9,101 3 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View