How could i use image j to count specific cells which are labeled by two FITC ab?

how should i adjust image j to count these cells automatically not manually? i put one of my slide image in attached file.

25 December 2020 9,598 2 View

Are polymer viscosity, storage and loss moduli volume-dependent?

It is welcomed to introduce any published paper in this respect.

26 November 2020 3,679 3 View

What tests should I run for this small scale research?

25 October 2020 5,690 3 View

Why can't I see band in gel agarose while 260/280 ratio is 1.8 in Nanodrop?

Hi everyone I am trying to extract DNA from soil, 260/280 ratio is 1.8 in nanodrop, concentration is 587 ng/ul. but I can not see the band in gel agarose. Thank you in advance

18 October 2020 9,438 7 View

I want to know to calculate green and blue water in SWAT model,if the data is entered on a daily basis, can I receive the output on a daily basis too?

Hi everyone need your help.thank you

22 September 2020 8,704 4 View

Does the alpha-synuclein overexpression in SH SY5Y cells have any toxic effect?

Cells are dying post-transfection. I've transfected SH SY5Y cells to overexpress alpha-synuclein, cells were overexpressed but they were dying rapidly. I've checked the overexpression, it was fine...

16 August 2020 7,518 3 View

What is the difference between the variables of TRMM (precipitation data) and which one I must use?

Hi everyone There is a screenshot of variables and I need to know what is the difference between number 1 to 6? and which one I must use in my work? thanks!

07 August 2020 4,432 3 View

Error while installing gromacs?

Hello all I was trying to install gromacs 2019 in an non-standard. but i countered this error: /usr/lib/gcc/x86_64-linux-gnu/5/include/mwaitxintrin.h(36): error: identifier...

16 June 2020 3,908 5 View

Number of Antennas for Massive MIMO (Commercially+ acc. to 5G Standardization)?

Currently, most of the reports show that the no. of Tx/Rx antennas for mMIMO (commercially deployed and tested) are upto128 (64T64R-TDD etc). Does anyone knows: 1. What is the number of Tx/Rx...

19 March 2020 3,987 8 View

Could you help me with simulating drag force and dielectrophoresis force in comsol by using AC/DC module?

I'm trying to apply a nonuniform electric field and create a dielectrophoretic force in a channel and put a particle in the middle of the channel. I added these 2 physics, creeping flow and...

13 March 2020 9,721 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Does any one can help me choose a reasonable basis set for intermediate metal ions interactions with guanine (i.e Fe2+, Mn2+, Cu2+ and exc.)?

Hello every body Does any one has any idea to help me choose a reasonable basis set for intermediate metal ions (i.e Fe2+, Mn2+, Cu2+ and exc.) interactions with guanine or other DNA organic...

01 March 2021 6,187 2 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

What is Flat Lens Antenna?

28 February 2021 5,433 3 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

How to use measure capacitance change in COMSOL time dependent study ?

I want to measure change of capacitance due to relative displacement between two parallel plates in COMSOL. Distance between two plates changes with time. So I'm using a time dependent study but...

26 February 2021 8,567 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View