Checking of sugarcane production and productivity by tissue culture method, I want to know the answer. As I read and study, this techniques couldn't create genomic modifications. Please, share your views?
Dear Ravindra Karn yes, it's a fact, and it's named somaclonal variation (see Larkin, P. J., & Scowcroft, W. R. (1981). Somaclonal variation—a novel source of variability from cell cultures for plant improvement. Theoretical and applied genetics, 60(4), 197-214.). Certainly, some morphogenic pathways are less prompted to form SV than other; e.g. axillary buds < meristems < adventitious buds < regeneration from calli
It doesn't create the variation per say, but, it does allow for propagation of existing somatic variation in your sample.
Which one creates more somaclonal variation? Plant tissue culturing or animal tissue culturing? And why?
Not always and it varies with the concentration of hormones used. Higher concentrations may be cause epigenetic changes but no tissue growth possible. Certain chemicals cause such changes but they are carcinogenesis in nature.
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