How to interpret flow cytometry results ?

Hi, I am working with biomaterials, and I did an analysis of cell viability with CFDA and one to mesure ROS with MitoSOX indicator. The software project 5 graphs (each for a different...

09 October 2017 1,558 4 View

How can I find out the area of a TEM micrograph?

I am working with size distributions of nanoparticles. But I also need to show my results as a function of area (#NP/area). Does anybody know how calculate the area of a TEM micrograph?

04 May 2014 6,971 4 View

How much UV-light exposure time is required to disinfect a laminar flow hood?

How much UV-light exposure time is required to disinfect a laminar flow hood?

06 July 2013 7,980 19 View

Which substrate is the best for Saintpaulia ionantha (african violet)?

Hi I am acclimating African violet, but I need help to choose the best substrate I have 4 options: 1.- peat moss 2.- peat moss (25%) & perlite (75%) 3.- peat moss (50%) & perlite (50%) 4.- peat...

06 July 2013 8,839 0 View

If you are measuring the interplanar spacing, what is the justified % of error to consider?

Hi I am measuring the interplanar spacing of some nanoparticles, but many of them are not exactly as the fcc that have to be. I look for an aproximate value to mine, But I like know what is...

06 July 2013 8,329 0 View

Explant, propagule or plant? How do you say....?

I am writing a protocol about the acclimatization of a plant But I don't know how to say : when I retire the culture of the medium, can I say that I obtain the plant, or is there another...

05 June 2013 1,110 1 View

Eucaryotic enzyme in lactobacillus plantarum

I want to insert papain which is a eucaryotic enzyme but contains 3 disulfure bonds. How can I do that such that lactobacillus plantarum expresses the enzyme with the correct structure?? I am...

04 May 2013 6,299 0 View

Fourier Transformation and lattice parameters.

I am working in the characterization of Silver nanoparticles, but I don't know exactly how to use my results of Fourier transformation specially in the angles and relate to silver lattice parameters.

04 May 2013 5,708 1 View

Optical properties for photoluminescence?

03 April 2013 480 2 View

A saccharomyces cerevisae vector with 35S promoter/

Does anybody know about a yeast plasmid that I could use to overexpress an ADH1 (Alcohol deshydrogenase 1) and that use a 35S enhancer?

02 March 2013 3,259 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is there a powerful system for the security of the systems distributed on IoT systems?

Is there a powerful system for the security of the systems distributed on IoT systems?

02 March 2021 3,858 10 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How Relativistic effects is one reason for lanthanide contraction?

The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...

01 March 2021 2,272 4 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View