Does anybody know about a yeast plasmid that I could use to overexpress an ADH1 (Alcohol deshydrogenase 1) and that use a 35S enhancer?
Why would you want 35S in yeast?
Maybe species of your yeast would be useful information.
We are going to use Saccharomyces cerevisiae, and I read that 35S is a strong promoter used in plants, but I don t know is there is a possibility to use in Sc (bacause my professor said that, I don´t know why) =/
Hi Ari,
your professor is right, 35S works in a broad variety of species and also in yeast (20 year old knowledge). Have a look on the added a publication and please see also: Mol Gen Genet (1992) 235:365 372. Try the addgene database or NCBI to find some vector that fits your needs best. HTH, Robert
I happened upon this 1990 paper and thought it might be of interest:
http://www.ncbi.nlm.nih.gov/pubmed/2202523
"Evolutionary conservation of transcriptional machinery between yeast and plants as shown by the efficient expression from the CaMV 35S promoter and 35S terminator."
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