How to interpret flow cytometry results ?

Hi, I am working with biomaterials, and I did an analysis of cell viability with CFDA and one to mesure ROS with MitoSOX indicator. The software project 5 graphs (each for a different...

09 October 2017 1,558 4 View

How can I find out the area of a TEM micrograph?

I am working with size distributions of nanoparticles. But I also need to show my results as a function of area (#NP/area). Does anybody know how calculate the area of a TEM micrograph?

04 May 2014 6,971 4 View

How much UV-light exposure time is required to disinfect a laminar flow hood?

How much UV-light exposure time is required to disinfect a laminar flow hood?

06 July 2013 7,980 19 View

Which substrate is the best for Saintpaulia ionantha (african violet)?

Hi I am acclimating African violet, but I need help to choose the best substrate I have 4 options: 1.- peat moss 2.- peat moss (25%) & perlite (75%) 3.- peat moss (50%) & perlite (50%) 4.- peat...

06 July 2013 8,839 0 View

If you are measuring the interplanar spacing, what is the justified % of error to consider?

Hi I am measuring the interplanar spacing of some nanoparticles, but many of them are not exactly as the fcc that have to be. I look for an aproximate value to mine, But I like know what is...

06 July 2013 8,329 0 View

Explant, propagule or plant? How do you say....?

I am writing a protocol about the acclimatization of a plant But I don't know how to say : when I retire the culture of the medium, can I say that I obtain the plant, or is there another...

05 June 2013 1,110 1 View

Fourier Transformation and lattice parameters.

I am working in the characterization of Silver nanoparticles, but I don't know exactly how to use my results of Fourier transformation specially in the angles and relate to silver lattice parameters.

04 May 2013 5,708 1 View

Optical properties for photoluminescence?

03 April 2013 480 2 View

A saccharomyces cerevisae vector with 35S promoter/

Does anybody know about a yeast plasmid that I could use to overexpress an ADH1 (Alcohol deshydrogenase 1) and that use a 35S enhancer?

02 March 2013 3,259 5 View

Does anyone have tables of lattice parameter?

I am trying to know the form of my nanoparticles of Ag, but I don't have the lattice tables, to compare the data of my fourier transform.

02 March 2013 7,094 8 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View