How to interpret flow cytometry results ?

More Areli Munive Olarte's questions See All

How can I find out the area of a TEM micrograph?

I am working with size distributions of nanoparticles. But I also need to show my results as a function of area (#NP/area). Does anybody know how calculate the area of a TEM micrograph?

04 May 2014 7,087 4 View

How much UV-light exposure time is required to disinfect a laminar flow hood?

06 July 2013 8,184 19 View

Which substrate is the best for Saintpaulia ionantha (african violet)?

Hi I am acclimating African violet, but I need help to choose the best substrate I have 4 options: 1.- peat moss 2.- peat moss (25%) & perlite (75%) 3.- peat moss (50%) & perlite (50%) 4.- peat...

06 July 2013 8,949 0 View

If you are measuring the interplanar spacing, what is the justified % of error to consider?

Hi I am measuring the interplanar spacing of some nanoparticles, but many of them are not exactly as the fcc that have to be. I look for an aproximate value to mine, But I like know what is...

06 July 2013 8,456 0 View

Explant, propagule or plant? How do you say....?

I am writing a protocol about the acclimatization of a plant But I don't know how to say : when I retire the culture of the medium, can I say that I obtain the plant, or is there another...

05 June 2013 1,212 1 View

Eucaryotic enzyme in lactobacillus plantarum

I want to insert papain which is a eucaryotic enzyme but contains 3 disulfure bonds. How can I do that such that lactobacillus plantarum expresses the enzyme with the correct structure?? I am...

04 May 2013 6,394 0 View

Fourier Transformation and lattice parameters.

I am working in the characterization of Silver nanoparticles, but I don't know exactly how to use my results of Fourier transformation specially in the angles and relate to silver lattice parameters.

04 May 2013 5,824 1 View

Optical properties for photoluminescence?

I am working with a software (Material´s Studio) to recognize the form of silver nanoparticles, but I would like to know theoretically by the results of the optical properties of the software if...

03 April 2013 584 2 View

A saccharomyces cerevisae vector with 35S promoter/

Does anybody know about a yeast plasmid that I could use to overexpress an ADH1 (Alcohol deshydrogenase 1) and that use a 35S enhancer?

02 March 2013 3,366 5 View

Does anyone have tables of lattice parameter?

I am trying to know the form of my nanoparticles of Ag, but I don't have the lattice tables, to compare the data of my fourier transform.

02 March 2013 7,216 8 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

Mebrahtu Tedla

can you display the the normal distribution of the cells in all the graphs?. from the histogram, all seems the same and hard to interpret.

Mona Dehhaghi

This file may help you:

https://fccf.epfl.ch/files/content/sites/facs/files/shared/interpreting%20flow%20cytometry%20data%20article.pdf

Han Zhang

Hi Areli, we need more information to interpret the FCS data. How did you gate the cells? And how about your negative control with no color?

Areli Munive Olarte

@Mebrahtu

the software display only this graphs