How to interpret flow cytometry results ?

Hi, I am working with biomaterials, and I did an analysis of cell viability with CFDA and one to mesure ROS with MitoSOX indicator. The software project 5 graphs (each for a different...

09 October 2017 1,558 4 View

How can I find out the area of a TEM micrograph?

I am working with size distributions of nanoparticles. But I also need to show my results as a function of area (#NP/area). Does anybody know how calculate the area of a TEM micrograph?

04 May 2014 6,971 4 View

How much UV-light exposure time is required to disinfect a laminar flow hood?

How much UV-light exposure time is required to disinfect a laminar flow hood?

06 July 2013 7,980 19 View

If you are measuring the interplanar spacing, what is the justified % of error to consider?

Hi I am measuring the interplanar spacing of some nanoparticles, but many of them are not exactly as the fcc that have to be. I look for an aproximate value to mine, But I like know what is...

06 July 2013 8,329 0 View

Explant, propagule or plant? How do you say....?

I am writing a protocol about the acclimatization of a plant But I don't know how to say : when I retire the culture of the medium, can I say that I obtain the plant, or is there another...

05 June 2013 1,110 1 View

Eucaryotic enzyme in lactobacillus plantarum

I want to insert papain which is a eucaryotic enzyme but contains 3 disulfure bonds. How can I do that such that lactobacillus plantarum expresses the enzyme with the correct structure?? I am...

04 May 2013 6,299 0 View

Fourier Transformation and lattice parameters.

I am working in the characterization of Silver nanoparticles, but I don't know exactly how to use my results of Fourier transformation specially in the angles and relate to silver lattice parameters.

04 May 2013 5,708 1 View

Optical properties for photoluminescence?

03 April 2013 480 2 View

A saccharomyces cerevisae vector with 35S promoter/

Does anybody know about a yeast plasmid that I could use to overexpress an ADH1 (Alcohol deshydrogenase 1) and that use a 35S enhancer?

02 March 2013 3,259 5 View

Does anyone have tables of lattice parameter?

I am trying to know the form of my nanoparticles of Ag, but I don't have the lattice tables, to compare the data of my fourier transform.

02 March 2013 7,094 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View