Is a multivariate linear mixed model appropriate to analyse differences over time?

Say I have a data set of 32 study participants. The dependent variables are two different physiological reactions to stimuli. These are repeated measures, recorded over 16 trials. There are two...

13 December 2020 7,218 3 View

Calculating effect sizes without knowing the sample size by subgroups?

Hello! I am calculating effect sizes for a research synthesis of the literature (e.g. Hedges' g), but many of the original publications do not provide the sample size (N) by subgroups. I see...

13 September 2020 7,587 3 View

Does anyone have a user manual for the Cahn DCA-322 Dynamic Contact Angle Analyser?

Our instrument is from about 1998 and we no longer have the manual. The Help function in the program needs to connect to the internet, but we have to keep the computer offline, therefore the help...

29 May 2019 6,109 3 View

Does anyone know of a dataset for seismic activity for Europe?

29 March 2019 4,501 3 View

How can I change my entry regarding my book?

I mistakenly wrote that my book would be open access. This is not the case and needs to be changed. Presumably this means that I cannot have reviews of it either?

07 March 2019 1,442 1 View

A problem with generation of lentiviral particles of a large gene (4kb)?

I am trying to generate lentiviral particles from a insert (4Kb) cloned into a lentiviral vector (pWPI, 11Kb) and I am having some issues. I transfected HEK 293T cells with the pMD2G, pPAX and our...

02 August 2018 5,170 3 View

What is the future of labor law in a future world without work?

Labor law is predominantly carried out from the domestic perspective of the countries through their legislation, accepting only some international (often disregarded) legislative...

23 March 2018 473 6 View

Hello everyone, I am wondering whether just from the EEG raw data alone you can identify where on the scalp the electrode is positioned?

In more detail, we are interested whether we can distinguish the HEOG electrode from the F7 electrode next to it? Thank you very much in advance and all the best, Miriam

24 September 2017 149 1 View

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13 June 2017 7,057 4 View

Which broth medium would be the equivalent to solid Standard Plate Count PCA??

I wish to analyze the bacterial composition in a food sample and I wanna use a liquid enrichment medium prior to agar media. I have been looking for but I am not sure which broth medium would...

12 June 2017 2,807 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What might be the reason for obtaining two bands in plant genomic DNA extraction?

I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.

21 February 2021 6,523 6 View

How to get the vector of a specific vibration mode in phonon dispersion?

For example, I have this phonon dispersion(Fig. 1), and now I would like get vector of atoms which describes TA1 in P. I know v_sim can show vibrational animation of specific mode, but I do not...

20 February 2021 4,332 4 View