We are performing the hemolysis assay in hydrogels which are more solid-like, we are wondering which size of the material should be used, and in which volume of the erythrocyte solution.
A typical reforming section in a large-scale Haber-Bosch process (Figure 1) comprises two reactors. A mixture of methane (CH4) and steam (H2O) is fed into a primary reformer to produce syngas via...
01 August 2024 7,051 5 View
I just joined to ResearchGate but I realized that several of my publications are linked to other "Marcos Andrade (Universidade Presbiteriana Mackenzie)". Please help me claim my work
14 May 2024 1,053 1 View
Electrochemistry is a pathway to convert CO2 into valuable products such as CO. However, a potential side product in the cathode is H2. I wonder if it is possible to tune the composition of the...
12 May 2024 9,061 3 View
text format and font
25 March 2024 4,678 3 View
Pulsed electric field is considered a "non-thermal" method for food processing that involves the application of short high-voltage pulses to create pores in cell membranes. However, since I am not...
13 March 2024 2,624 6 View
I read that potassium clavulanate is water soluble. I tried to dissolve it to a very low concentration but the liquid remains cloudy and a white powder is visible at the bottom of the vial
06 March 2024 8,900 0 View
Dear smart friends, I have a question about sonification. Can you advise me how I can convert human movement recorded by inertial sensors into sound? Is there any available program/application to...
07 February 2024 5,413 3 View
I've seen different concepts in various articles, where they mention "maintaining 50% field capacity" or around 30% humidity. But how do I get to that information?
30 January 2024 8,168 2 View
Hello! I was working with mice primary cell cultures to evaluate in vitro cytotoxicity of a treatment, I have an n of 10. The problem is that I don't know what technique to use because when I try...
27 January 2024 6,207 1 View
Good Day! I'm Margienalyn S. Flores. A Master of Arts in Educational Management. May I ask a question regarding your study because there are some parts of it that I'm interested in, "What role...
09 January 2024 399 7 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View