I'm wondering about media usage in iPSC culture. Most papers/protocols work with mTESR1, but for me it seems that PluriSTEM needs less media change and no overweekend change.
Does anyone use PluriSTEM with matrigel/ECM and has good results?
and btw: Does EDTA-solution or EDTA-based solution (like Versene) for cell passaging also work on matrigel/ECM?
I am not familiar with PluriSTEM, but I can share my experience with the mTeSR family products.
Currently, we utilise mTeSR Plus in our laboratory, which is formulated to prevent frequent changes in the culture medium and to ensure safety over the weekend.
Best wishes!
EDTA at 0.5 mmol/L works fine to passage iPSCs cultured in Matrigel-coated plates.
I'm working with Essential 8™ Flex Medium Kit, and it also has 2 days break on weekends.
Hi,
in our lab we use mTESR1 only for very sensitive cell lines. Otherwise we use iPS Brew, which works really well and is cheaper. I even feed only every second day with that and my cells are happy. This should be tested for each cell line though.
For passaging we use 0.5mM EDTA, like mentioned before.
Good luck with your cell culture!
Selene
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