Hi researchers I am looking for invitro assays for Parkinsons Disease like invitro assay for we are doing anti diabetic test using PTP1B assay if anyone know please send share with me. Looking forward to have good responses and thanking in advance
The simple answer is that there are none!
Parkinson's is best thought of as a spectrum of conditions, closely related by a gradual and progressive loss of dopaminergic neurons, but manifested in different combinations of symptoms. In this respect it is more analogous to autism than to Alzheimer's disease. As far as I know there are no defining symptoms and no single objective way of measuring disease progression. I personally have at least six symptoms that are unmistakeably typical of Parkinson's but I lack tremor, depression, REM sleep disturbance, cognitive impairment and anosmia. So I say I have PSD (Parkinsons Spectrum Disorder).
The Fox Foundation supports an organisation, PPMI, that looks for markers for PD, mainly for the purpose of assessing disease progress. You can:
Visit Parkinson's Progression Markers Research at http://www.ppmi-info.org/
23andMe, a US internet research site, has a worldwide database of 10 000 15 000 individuals, mostly with PD, and is looking for genetic associations. You can find it at https://www.23andme.com/en.
http://en.wikipedia.org/wiki/23andMe gives a clearer idea of what the company does now and you can see that it has diversified into more lucrative and risky areas. I joined in 2011 when PD patients were free!
It depends a bit on what you want. If you want something to test pathological specimens, like brain slices, then I would recommend alpha-synuclein staining. But if you are looking for an in vivo assay of disease progression, this will be difficult. Nowadays some rely on the progression of imaging markers, such as the ones used in nuclear imaging of the dopaminergic system. There are some trials with synuclein in CSF which seem to be promising.
Yes, there is a new in vitro PD assay, induced my misfolded alpha-synuclein oligomers in primary striatal or cortical neurons from rat or mice. Please look at www.synaging.com for more information.
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