How to quantify/calculate GC-MS analyzed data using isotope labelled (d3) standard?

Dear researchers I would like to learn how to quantify extract component A. I run extract at concentration of (0.2815 mg/ml). The extract was spiked with isotope labelled deuterated (A-d3)...

04 May 2019 5,839 1 View

Unwanted negative peaks in HPLC chromatogram ?

Dear fellows I am working on HPLC and run sample (mixture of 5 compounds) all were detected perfectly, but I am getting unwanted negative peaks at 2 mins after run? Can anyone figureout the issue?...

07 August 2018 8,656 1 View

Are there any diabetes marker cell lines?

I want to check the effect of different plant extracts using diabetic marker cell lines if anyone know kindly share information. Thanking in advance

03 April 2015 9,014 3 View

Anyone know about the best/optimum ratio of cells which is used for LPS-induced Nitric oxide production in RAW 264.7 cells using 24 well plate?

I am evaluating the anti-inflammatory effect of plant extracts and performed the experiment many times using RAW 264.7 cells but could not get the significant results. I want to know that how much...

02 March 2015 8,643 4 View

Can I use EtOAc:MeOH:Water in ratio of (100:20:1) for Sephadex LH-20? IF not then which solvent can I use as mobile phase for Sephadex LH-20 column?

I am isolating compounds from BuOH fraction and initially I did Silica gel column with mobile solvent was (EMW in 100:20:1) now I have plan to proceed with Sephadex LH-20 column. I want to know...

02 March 2015 183 4 View

First concentration (100 uL/ml) has lower average value while lower concentration (50 uL/ml) has higher average value? anyone know where error in exp?

11 December 2014 6,371 1 View

Does anyone know how to isolate compounds from Brassica oleracea group having n-Buoh fraction?

11 December 2014 9,484 6 View

Does anyone know an in-vitro protocol for inhibition of Monoamine Oxidase-A and B? can you send Protocol or share some useful articles?

10 November 2014 8,776 3 View

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10 November 2014 7,962 3 View

Please share protocol to measure oral glucose tolerance test in animal models??

Hi, researchers I want to perform antidiabetic tests in animals and want to measure the oral glucose tolerance test if anybody knows please share it with me. Answers and comments will be highly...

10 November 2014 7,796 1 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View