How to quantify/calculate GC-MS analyzed data using isotope labelled (d3) standard?

Dear researchers I would like to learn how to quantify extract component A. I run extract at concentration of (0.2815 mg/ml). The extract was spiked with isotope labelled deuterated (A-d3)...

04 May 2019 5,839 1 View

Unwanted negative peaks in HPLC chromatogram ?

Dear fellows I am working on HPLC and run sample (mixture of 5 compounds) all were detected perfectly, but I am getting unwanted negative peaks at 2 mins after run? Can anyone figureout the issue?...

07 August 2018 8,656 1 View

Are there any diabetes marker cell lines?

I want to check the effect of different plant extracts using diabetic marker cell lines if anyone know kindly share information. Thanking in advance

03 April 2015 9,014 3 View

Anyone know about the best/optimum ratio of cells which is used for LPS-induced Nitric oxide production in RAW 264.7 cells using 24 well plate?

I am evaluating the anti-inflammatory effect of plant extracts and performed the experiment many times using RAW 264.7 cells but could not get the significant results. I want to know that how much...

02 March 2015 8,643 4 View

Can I use EtOAc:MeOH:Water in ratio of (100:20:1) for Sephadex LH-20? IF not then which solvent can I use as mobile phase for Sephadex LH-20 column?

I am isolating compounds from BuOH fraction and initially I did Silica gel column with mobile solvent was (EMW in 100:20:1) now I have plan to proceed with Sephadex LH-20 column. I want to know...

02 March 2015 183 4 View

Does anyone know how to isolate compounds from Brassica oleracea group having n-Buoh fraction?

11 December 2014 9,484 6 View

Does anyone know how to check the activity of the plant extracts against Parkinson's disease for the in vitro models?

10 November 2014 7,460 3 View

Does anyone know an in-vitro protocol for inhibition of Monoamine Oxidase-A and B? can you send Protocol or share some useful articles?

10 November 2014 8,776 3 View

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10 November 2014 7,962 3 View

Please share protocol to measure oral glucose tolerance test in animal models??

Hi, researchers I want to perform antidiabetic tests in animals and want to measure the oral glucose tolerance test if anybody knows please share it with me. Answers and comments will be highly...

10 November 2014 7,796 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Do you know of an informal caregiver of a person who has recently received a bone marrow transplant?

We are conducting a pilot study of an intervention for informal caregivers of persons who have recently received a bone marrow transplant in the US. If you know of anyone who might be interested,...

13 February 2021 9,174 1 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Concentration of DNA?

Hello everyone! Is a normal value 295.8 ug/ul for cDNA? If not what does it mean? Where is the mistake? The measurement was done with nano drop. Thank you!

09 February 2021 6,202 1 View

What is the reference value of THD in BLDC MOTOR speed control ?

i used to control EHO+ANFIS to MRPID to Landsman converter for bldc drive i got 7% but some of them are telling IEEE standards are < 5% is it correct for Drives ?

07 February 2021 5,892 1 View

Does Location Map of Informal Settlements in South Africa exist?

I urgently need a map that shows the point locations of informal settlements in South Africa. I am currently working on a study that seeks to relate protests location to the location of informal...

02 February 2021 4,113 7 View

Is it possible estimating environmental conditions with a Bayesian approach?

In ecology specific species can be used to get an indication/estimation of the environmental conditions. A simplistic example, lets assume I find a pike (Esox lucius). We know this species does...

01 February 2021 10,034 4 View

How to know the threshold limit value of a sar image...??

i want to do thresholding by segregation of water areas from non water areas for that i am using model maker in ERDAS but i am unable to get the threshold limit value of my image.

26 January 2021 8,277 1 View