Zaheer Ahmad

17 Questions 4 Answers 0 Followers

Questions related from Zaheer Ahmad

How to quantify/calculate GC-MS analyzed data using isotope labelled (d3) standard?

Dear researchers I would like to learn how to quantify extract component A. I run extract at concentration of (0.2815 mg/ml). The extract was spiked with isotope labelled deuterated (A-d3)...

05 May 2019 5,937 1 View

Unwanted negative peaks in HPLC chromatogram ?

Dear fellows I am working on HPLC and run sample (mixture of 5 compounds) all were detected perfectly, but I am getting unwanted negative peaks at 2 mins after run? Can anyone figureout the issue?...

08 August 2018 8,762 1 View

Are there any diabetes marker cell lines?

I want to check the effect of different plant extracts using diabetic marker cell lines if anyone know kindly share information. Thanking in advance

04 April 2015 9,113 3 View

Can I use EtOAc:MeOH:Water in ratio of (100:20:1) for Sephadex LH-20? IF not then which solvent can I use as mobile phase for Sephadex LH-20 column?

I am isolating compounds from BuOH fraction and initially I did Silica gel column with mobile solvent was (EMW in 100:20:1) now I have plan to proceed with Sephadex LH-20 column. I want to know...

03 March 2015 264 4 View

Anyone know about the best/optimum ratio of cells which is used for LPS-induced Nitric oxide production in RAW 264.7 cells using 24 well plate?

I am evaluating the anti-inflammatory effect of plant extracts and performed the experiment many times using RAW 264.7 cells but could not get the significant results. I want to know that how much...

03 March 2015 8,723 4 View

First concentration (100 uL/ml) has lower average value while lower concentration (50 uL/ml) has higher average value? anyone know where error in exp?

Hi, I used basically three concentration to check their anti-inflammatory effects but surprisingly my first (100 uL/ml) showed lower average value than the 2nd concentration (50 uL/ml). Anyone...

12 December 2014 6,456 1 View

Does anyone know how to isolate compounds from Brassica oleracea group having n-Buoh fraction?

Hi, I want to isolate compounds from Brassica oleracea pants having n-Buoh fraction. I tried many solvents but could not get the desired results even the TLC plates did not showing the separated...

12 December 2014 9,589 6 View

Does anyone know an in-vitro protocol for inhibition of Monoamine Oxidase-A and B? can you send Protocol or share some useful articles?

Hi, all researcher I want to check activity of plant extracts against Monoamine Oxidase-A and B. If someone have its few or detailed protocol kindly send it to me. Every single effort will be...

11 November 2014 8,877 3 View

Does anyone know an invitro assay for Parkinson's Disease, like Acetylcholineasterase assay for Alzheimer's and PTP1B for diabetes?

Hi researchers I am looking for invitro assays for Parkinsons Disease like invitro assay for we are doing anti diabetic test using PTP1B assay if anyone know please send share with me. Looking...

11 November 2014 4,761 5 View

Please share protocol to measure oral glucose tolerance test in animal models??

Hi, researchers I want to perform antidiabetic tests in animals and want to measure the oral glucose tolerance test if anybody knows please share it with me. Answers and comments will be highly...

11 November 2014 7,869 1 View

Does anyone know how to check the activity of the plant extracts against Parkinson's disease for the in vitro models?

I am looking for the protocol to evaluate activity of plant extract against Parkinson's disease in in vitro models. If someone have any idea about this, please share with me.

11 November 2014 7,538 3 View

How can one evaluate the anti-oxidant effects of plants in animal models?

I want to check plant extract as potential source of anti-oxidants in animal models, please can someone recommend a protocol for this. Thanks for time given to read this question.

11 November 2014 8,412 4 View

What is a common cytotoxic agent and how does it induce cytotoxicity in SH-SY5Y to assess the cytoprotective effect of plant for Parkinson’s disease?

Hi researchers, I am evaluating the plant extracts against Parkinson's Disease. I want to know which is the common cytotoxic agent to induce cytotoxicity in SH-SY5Y neuroblastoma cells. If...

11 November 2014 8,026 3 View

How do you induce diabetes using streptozotin in rats?

I want to evaluate the effect of plant extracts against diabetes in animal models. I need a protocol for this experiment. Please help me by giving me the protocol. Thanks for the time given to...

11 November 2014 5,153 4 View

How do I pipet and handle a 96 well plate, previously I performed a PTP1B experiment using a 96 well plate but all the values were negative?

Hi all researchers, I want to perform PTP1B experiments. Previously I performed this experiment and analyzed data but all the values were negative. Someone told me that my pipetting is not good,...

10 October 2014 3,009 4 View

What is the best brain cell lines to study the acetylcholine and butyrylcholine inhibitory assays?

I want to work with brain cell lines which are best to study the effects of different compounds as acetylcholine and butylcholine inhibitors. so far I do not have any experience of working with...

09 September 2014 8,897 5 View

Which solvents and their ratio I need to use to isolate the compounds from the n-BuOH and H20 fraction using column chromatography?

I am isolating compounds from plants sources. I have five fractions (MeOH, Dichloromethane, Ethyl acetate, n-BuOH and H20). Now I am using two last fractions to isolate compounds i. e. n-BuOH and...

09 September 2014 2,526 1 View