Macrophages in culture not growing/ dying or detaching . Why ?

Hello, I am an undergraduate student doing my dissertation, where I isolate PBMCs from leucocyte cones in order to plate monocytes and allow for macrophage differentiation. Although the numbers...

09 February 2021 5,334 8 View

From your experience - what are the biggest impediments in the implementation of Digital Twins?

Even almost 20 years after the first Digital Twin (DT) concept was presented by NASA, there is still no broad implementation of DTs in industry is known to date. Why is that? What is hindering...

29 October 2020 1,003 7 View

Do you know of any publication manuals or style guides that have a chapter on bias-free / inclusive language, aside from APA (7th edition)?

The Publication Manual of APA (7th edition) has a very useful chapter on bias-free language. I would like to know if you've come across such chapters or sections in other publication manuals or...

21 October 2020 1,582 4 View

What can I do to get a straight baseline in RP-HPLC?

I am running a RP-HPLC system with a C18 column. In our group, we mainly use the system to analyze peptides. The baseline of the system is not stable. In every run it is increasing over time. The...

29 July 2020 5,003 11 View

What is the optimal well surface area for C2C12 myoblast differentiation into myotubes?

Does anyone know of studies investigating the optimal well sizes for growth/hypertrophy of C2C12 myoblast-derived myotubes following differentiation using standard serum starvation? I find that...

06 July 2020 598 2 View

Which tube should I use to connect vacuum oil pump with Schlenk line?

I want to connect the vacuum oil pumps in our lab with the Schlenk lines and I am not sure which tubes (material, brand, etc.) I should choose. At the moment I am using a red rubber tube and it is...

30 January 2020 7,354 2 View

Superficiality or shallowness scales for trait evaluation?

Does anyone know if there exist a psychometric scale that measures the personal shallowness/superficiality of an individual when evaluating other's individual traits (age, race, gender,...

11 September 2019 2,566 3 View

How to calculate inter-coder reliability (two coders) with observations fitting almost into the same category?

02 June 2019 979 2 View

HT22 purchase in Greece?

Hello everybody, I am looking for a specific cell line HT22 but I haven't found any company that could import it in Greece. Has anyone faced that problem before? Is there any alternative way that...

01 April 2019 1,917 3 View

Hello, Can anyone help me use krona tool for visualising my NGS data?

My files contain data in percentages or in counts.Does Krona tool need bioinformatic language to be used? Thanks in advance.

27 January 2019 1,758 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View