Why is methylene blue commonly used to measure an active surface area of nanoparticles?

Among the dyes, why is methylene blue commonly used to measure an active surface area of nanoparticles(especially graphene derivatives)? I try to find the reason, but I cannot find it. Could you...

11 March 2020 2,963 4 View

What is the difference between using Matlab or SUMO(with TraCI) in urabn traffic simulation?

Hello friends, I am a beginner in urban traffic simulation. I searched on the internet about traffic simulators in days ago. I am familiar to Matlab and I found that Matlab can be used in VANET...

18 July 2019 8,431 2 View

How to I create a stock solution of Curcumin in DMSO?

Curcumin (368.385 g/mol). DMSO purity 99.99% Curcumin solubility in DMSO (25 mg/ml), I need to create a stock concentration of 40mM. How much of each do I need? Can someone please explain how...

13 March 2019 6,327 4 View

What are some good examples of articles for moderately polar compounds separated by HILIC?

LogP

20 September 2017 5,023 4 View

Are there any infra red detectors for HPLC or UV+IR detector for liquid or gas or supercritical fluid chromatography?

Just wondering about their expense to make. Does liquid propulsion cause excess interference in IR?. A multifunctional HPLC that checks for impurities would be more valuable. Does the liquid...

07 September 2017 3,555 4 View

How to dissolve acidic peptide?

Hi all, I know there have been questions and answers on how to dissolve an acidic peptide. However, my questions will be: a) Can I dissolve my peptide using a basic solvent such as NaOH instead of...

02 September 2017 4,042 5 View

Phenazines and pyocyanin contain a chromophore. What makes it contain a chromophore in its structure?

I know benzene rings in tryptophan at 280nm give the absorption so is this the only criterion for a molecule to possess a chromophore?. Or do the functional groups play a pivotal role along with...

29 August 2017 3,081 5 View

How efficient in plate count are gaurd columns on their own such as C18 based gaurd column or one of a different material such as ion exchange/ HILIC?

"A properly designed guard column does not deteriorate the separation. As a matter of fact, a well designed and well packed guard column using the same material as in the analytical column is a...

26 August 2017 6,915 12 View

What is the sensitivity of hplc uv DAD detector compared the the BDD electrode?

The Boron doped diamond electrode is very sensitive. I need to know how hplc uv DAD compares so that I can perform easy Limit of detection testing without making up too many samples, and even...

26 August 2017 185 4 View

What statistical method do I use to calculate HPLC resolution?

25 August 2017 1,613 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View