Mel Ro

3 Questions 1 Answers 0 Followers

Questions related from Mel Ro

Does anyone have experience with RPM for HEK Suspension Culture?

I am trying to find out the recommended RPM for growing suspension HEK293f cells (Invitrogen). I am growing them at a 500ml volume in a 2L flask (BD).

20 August 2014 387 1 View

Can anyone answer some questions regarding stable cell lines- CHO?

We have created a stable cell line with CHO DG44 cells. I have a couple of questions. 1. Do we need to continue MTX selection during passaging after clonal selection? 2. Even after clonal...

15 August 2014 7,066 3 View

Can anyone suggest what might be going wrong with a Transient Expression- HEK293?

We complete transient transfections with HEK293 cells. Recently we have seen a dramatic decrease in protein expression with proteins we have expressed times before. We've checked the equipment for...

15 August 2014 1,336 2 View