If so, how expired were your components and how did your results turn out?
I am purifying a His-tagged protein from an EColi expression system. After the purification I am doing an activity assay so it is vital that I get active protein. First, it seems that as my...
06 July 2015 6,205 8 View
If so, how long did it take for single cell to grow and what growth medium composition did you use?
31 December 2014 7,195 1 View
I am looking for a dilution protocol to make a monoclonal cell line from NIH-3T3 cells. Basically, what I would like to know is: 1.) When you have a single cell isolated what growth...
31 December 2014 2,860 1 View
I am doing a western of a protein that is encoded in the nucleus but is then imported into the mitochondria. The immature protein contains a mitochondrial targeting sequence that is cleaved upon...
06 July 2014 5,666 4 View
I am looking for a bioinformatic way to predict protein structural changes when I acetylate a specific residue of a protein. Any suggestions?
04 May 2014 8,205 4 View
I am trying to introduce 7 point mutations into a 23bp sequence. I have experience doing single point mutations via quikchange site-directed mutagenesis. I was wondering how many point mutations...
04 May 2014 5,539 2 View
I currently use TOPO cloning to identify cell populations that have indels from CRISPR. I have also used SURVEYOR mismatch assay. Sometimes I can use genotyping PCR and see bands of different...
01 January 1970 5,801 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates
03 August 2024 3,505 6 View