Julie Lynn Horton

8 Questions 23 Answers 0 Followers

Questions related from Julie Lynn Horton

Why does my purified protein have a pellet when I spin it down?

I am purifying a His-tagged protein from an EColi expression system. After the purification I am doing an activity assay so it is vital that I get active protein. First, it seems that as my...

07 July 2015 6,161 8 View

Does anyone have a NIH3T3 monoclonal cell line production protocol?

I am looking for a dilution protocol to make a monoclonal cell line from NIH-3T3 cells. Basically, what I would like to know is: 1.) When you have a single cell isolated what growth...

01 January 2015 2,831 1 View

Has anyone made a clonal cell line using C2C12 cells?

If so, how long did it take for single cell to grow and what growth medium composition did you use?

01 January 2015 7,162 1 View

Do mitochondrial proteins encoded by the nucleus have two bands on western?

I am doing a western of a protein that is encoded in the nucleus but is then imported into the mitochondria. The immature protein contains a mitochondrial targeting sequence that is cleaved upon...

07 July 2014 5,626 4 View

How many sites have you mutated in a single run of site-directed mutagenesis?

I am trying to introduce 7 point mutations into a 23bp sequence. I have experience doing single point mutations via quikchange site-directed mutagenesis. I was wondering how many point mutations...

05 May 2014 5,508 2 View

What software can I use to predict protein structural changes?

I am looking for a bioinformatic way to predict protein structural changes when I acetylate a specific residue of a protein. Any suggestions?

05 May 2014 8,173 4 View

Does anyone have experience using expired seahorse calibration plates or cell culture plates?

If so, how expired were your components and how did your results turn out?

04 April 2014 1,617 0 View

What is the most efficient method to screen cells for indels made when using CRISPR/Cas9?

I currently use TOPO cloning to identify cell populations that have indels from CRISPR. I have also used SURVEYOR mismatch assay. Sometimes I can use genotyping PCR and see bands of different...

01 January 1970 5,767 3 View