I currently use TOPO cloning to identify cell populations that have indels from CRISPR. I have also used SURVEYOR mismatch assay. Sometimes I can use genotyping PCR and see bands of different sizes on a gel. This doesn't always work, though, as small indels are difficult to resolve. Has anyone developed a quicker, less expensive way to identify cells with indels?
I'm usind the T7E1 assay, it's faster and cheaper compared to the Surveyor assay and more sensitive if you're looking for small indels!
I am using the heteroduplex assay don't kow how if quantitative but works fine
I used the T7E1 assay ( that works similarly but better than the surveyor nuclease for me, and is way cheaper ) and ran it on polyacrylamide gel. It was quite sensitive and enabled me to choose between different possible sgRNAs
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