Why does my purified protein have a pellet when I spin it down?

06 July 2015 6,049 8 View

Does anyone have a NIH3T3 monoclonal cell line production protocol?

I am looking for a dilution protocol to make a monoclonal cell line from NIH-3T3 cells. Basically, what I would like to know is: 1.) When you have a single cell isolated what growth...

31 December 2014 2,762 1 View

Do mitochondrial proteins encoded by the nucleus have two bands on western?

06 July 2014 5,547 4 View

What software can I use to predict protein structural changes?

I am looking for a bioinformatic way to predict protein structural changes when I acetylate a specific residue of a protein. Any suggestions?

04 May 2014 8,095 4 View

How many sites have you mutated in a single run of site-directed mutagenesis?

I am trying to introduce 7 point mutations into a 23bp sequence. I have experience doing single point mutations via quikchange site-directed mutagenesis. I was wondering how many point mutations...

04 May 2014 5,435 2 View

Does anyone have experience using expired seahorse calibration plates or cell culture plates?

If so, how expired were your components and how did your results turn out?

03 April 2014 1,497 0 View

What is the most efficient method to screen cells for indels made when using CRISPR/Cas9?

I currently use TOPO cloning to identify cell populations that have indels from CRISPR. I have also used SURVEYOR mismatch assay. Sometimes I can use genotyping PCR and see bands of different...

01 January 1970 5,696 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View