I have isolated svf from adipose tissues and will be characterising the macrophage using facs. I need to grow the isolated macrophages and need optimised protocol.
Hello.
Hope be useful for you:
1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6391981/#:~:text=Isolate%20the%20Stromal%20Vascular%20Fraction,cells%20pelleted%20on%20the%20bottom.
2. https://bnrc.springeropen.com/articles/10.1186/s42269-022-00747-7
3. https://www.jove.com/t/50707/isolation-of-adipose-tissue-immune-cells
4. https://link.springer.com/protocol/10.1007/978-1-4939-9195-2_18
5.
https://link.springer.com/article/10.1007/s10616-020-00407-6
6. https://www.google.com/url?sa=t&source=web&rct=j&url=https://www.biorxiv.org/content/10.1101/336032v1.full.pdf&ved=2ahUKEwiP9YqYoa_5AhWji_0HHSQ4A7M4ChAWegQIHBAB&usg=AOvVaw2hq8TcJGN37cMZnFeGzz86
Best wishes
F. Nazari
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