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Questions related from Sy Alee
I need to determine indentation and tissue stiffness. I already have paraffin embedded tissue, but not sure if that will give an accurate result or I should use a fresh tissue.
12 February 2024 2,103 0 View
I have to determine adipose tissue stiffness. My samples were preserved in NBF. Can I use those tissue sections? or should I use fresh tissue to identify tissue stiffness? Is there any protocol...
02 February 2024 7,865 0 View
I have to characterise fibroblasts and preadipocytes from fat tissue. MOst of the articles have used DMEM/ F12, while some have used DMEM. Is there any rational behind using specific media over...
22 November 2023 4,229 0 View
I am sorting preadipocytes from SVF and now need to differentiate them into mature adipocytes. Does a mixed cocktail of preadipocyte differentiation media works best of making a cocktail by...
08 October 2023 1,103 0 View
I have to differenitate stem cells into preadipocyte and further into mature adipocyte. We want to quantify ECM produced by adipocytes. Not sure if I should use Immunoflurence or there is any...
05 September 2023 6,440 0 View
What's the hallmark of adipose tissue fibrosis? What techniques can be performed to identify and quantify adipose tissue fibrosis?
25 July 2023 2,686 0 View
I have to characterize fibroblast and myofibroblast in adipose tissue. Since these are connective tissue cells and ubiquitously present in all connective tissue therefore, isolation from a...
19 June 2023 1,292 1 View
I have got nichenet data which will be used to select highly interacting ligand-receptor pairs for functional analysis. the ligands from the sender cells are represented by LogFC while the...
04 June 2023 7,409 0 View
I have to determine phenotype of macrophages M1 or M2 in SVF obtained from adipose tissue. Which technique would be relevant to identify macrophage phenotype flow cytometry (using surface markers)...
16 April 2023 1,211 3 View
Im doing flow cytometry using 8 different antibodies. I have to optimize the protocol before i run my final samples. I have to use zombie UV which requires PBS (no serum) washing of cells before...
23 January 2023 1,561 5 View
I have a long list of genes, some are receptors and some are ligands. Based on our single cell RNA sequencing I have that data on up and down regulation of these genes. Is their any software that...
22 January 2023 5,549 0 View
I'm looking for bioinformatics softwares to determine intercellular interactions between macrophages and endothelial cells, the pathways and genes involved. Please recommend any software that can...
24 December 2022 1,160 1 View
I have scRNA sequencing data and I want to perform differential gene expression in macrophages. However, I want to include bioinformatics that could complement my studies. Is there any tool that I...
24 December 2022 4,899 4 View
We have scRNA sequencing data and we have to short the genes of interest. I have tried cellphonedb but the list was not very impressive. There are about 400 genes we have short-listed on the basis...
20 November 2022 3,037 0 View
We have to differentiate HSC to macrophages and we just got one vial from ATCC. Which growth factor would be more appropriate for macrophage differentiation? After thawing the HSC, should we...
08 November 2022 999 1 View
I have isolated svf from adipose tissues and will be characterising the macrophage using facs. I need to grow the isolated macrophages and need optimised protocol.
04 August 2022 6,215 1 View
