Sy Alee

8 Questions 5 Answers 0 Followers

Questions related from Sy Alee

How to identify adipose tissue fibrosis?

What's the hallmark of adipose tissue fibrosis? What techniques can be performed to identify and quantify adipose tissue fibrosis?

25 July 2023 2,680 0 View

How do you identify myofibroblast being responsible for adipose tissue fibrosis?

I have to characterize fibroblast and myofibroblast in adipose tissue. Since these are connective tissue cells and ubiquitously present in all connective tissue therefore, isolation from a...

19 June 2023 1,283 1 View

How do you analyze cell interactome data and make informed decisons?

I have got nichenet data which will be used to select highly interacting ligand-receptor pairs for functional analysis. the ligands from the sender cells are represented by LogFC while the...

04 June 2023 7,402 0 View

Which technique would be relevant to identify macrophage phenotype flow cytometry (using surface markers) or ELISA?

I have to determine phenotype of macrophages M1 or M2 in SVF obtained from adipose tissue. Which technique would be relevant to identify macrophage phenotype flow cytometry (using surface markers)...

16 April 2023 1,205 3 View

UV zombie requires washing with PBS (with no BSA) and FC block is prepared in stain buffer containing BSA. Should I wash in between?

Im doing flow cytometry using 8 different antibodies. I have to optimize the protocol before i run my final samples. I have to use zombie UV which requires PBS (no serum) washing of cells before...

23 January 2023 1,556 5 View

Which bioinformatics softwares can be used to determine intercellular interactions between immune and non immune cells?

I'm looking for bioinformatics softwares to determine intercellular interactions between macrophages and endothelial cells, the pathways and genes involved. Please recommend any software that can...

24 December 2022 1,157 1 View

Which Bioinformatics tools can be used to validate scRNA sequencing data?

I have scRNA sequencing data and I want to perform differential gene expression in macrophages. However, I want to include bioinformatics that could complement my studies. Is there any tool that I...

24 December 2022 4,889 4 View

Does anyone has protocol to culture macrophages from.adipose tissues?

I have isolated svf from adipose tissues and will be characterising the macrophage using facs. I need to grow the isolated macrophages and need optimised protocol.

04 August 2022 6,204 1 View