Reagents and protocol for transient transfection in meduloblastoma cell lines?

does anyone can suggest reagents and protocols for transient transfection (pcDNA 3.1 plasmid) in meduloblastoma cell lines (daoy, d283, d425, d458 and hd-mb03)? We normally use PEI for transient...

06 July 2019 8,947 2 View

Anyone using BIOCOLOR life science assays?

We recently bought 3 kit to measure collagen and elastin in cells... The manuals are very confusing, badly written with sparse informations... But the worst is to contact costumer/technical...

08 September 2018 2,927 3 View

Does anyone know which mutations the cell lines Huh7, Hep G2, Hep 3b have?

We basically need to know if the cell lines have PIK3CA activation. Nonetheless, is not easy to find informations in ATCC website. Thank you for your help. best

09 October 2016 4,246 4 View

How to remove non-tumour cells from a mouse tumour?

We have a breast cancer mouse model from which we want to extract the tumour, dissociate in single cells and then remove all non-tumour cells to have a pure population of tumour cells to inject in...

09 October 2016 2,155 2 View

Can someone recommended antibodies to perform a co-staining (IHC-P) of CD44/CD24 in paraffin embedded sections (mouse samples)?

07 August 2016 8,613 2 View

Does anyone added NAD+ (or any precursor) in culture media?

02 March 2016 7,507 3 View

Did anyone used an inducible system that express rtTA ubiquitously in transgenic mice?

We are engaged in a metabolic project where we want to express our gene in a whole body and inducible fashion (or at least in important metabolic tissues as muscle, WAT, BAT and liver). Do anyone...

31 December 2014 5,256 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View