We are engaged in a metabolic project where we want to express our gene in a whole body and inducible fashion (or at least in important metabolic tissues as muscle, WAT, BAT and liver).
Do anyone know a model to express rtTA ubiquitously?
Thanks
Hi Rafael,
Yes we use Rosa26-rtTA line often. It is a conditional one so you can use it with your favorite cre line or use a ubiquiteous cre to delete floxed stop cassette in the germline and use it as a ubiquiteous rtTA expressing line.
Pub; Belteki et al, 2005 NAR 33(5):e51
Best,
Steven
Hi Steven,
Thanks for your answer.
I was thinking about ROSA26 but it seems not well express in whole body. Am I wrong?
Rosa26 is one of the few loci that are really ubiquiteously expressed and expression is similar in most tissues/cell types which is an advantage ... However, the rosa26 promoter is a very moderate promoter and transgene expression is rather low (compared to a CMV promoter). But for rtTA this should not be a problem . And you even can breed them to homozygocity, doubling the amount of the rtTA trangene, if you think this will be a problem (but we never had to do this).
Expression of transgene rtTA from an exogeneous promoter like CMV will yield much higher expression but expression is very variable as these promoters often get silenced in certain cell types and conditions depending on the locus where they get integrated.
If we do not see expression from a dox-inducible transgene expression system it is rather due to silencing of the tetO promoter element than due to levels of rtTA, so I would rather worry about this.
Hi, I would look at this line: http://jaxmice.jax.org/strain/016532.html
reference: Premsrirut PK; Dow LE; Kim SY; Camiolo M; Malone CD; Miething C; Scuoppo C; Zuber J; Dickins RA; Kogan SC; Shroyer KR; Sordella R; Hannon GJ; Lowe SW. 2011. A rapid and scalable system for studying gene function in mice using conditional RNA interference. Cell 145(1):145-58. [PubMed: 21458673] [MGI Ref ID J:171191]
However, in this paper the line is only shortly described. But as far as I know it is the best one for broad expression.
You can also have a look here:
http://www.tetsystems.com/support/transgenic-rodent-lines
regards-
kai
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