Rafael Ioris

8 Questions 9 Answers 0 Followers

Questions related from Rafael Ioris

Reagents and protocol for transient transfection in meduloblastoma cell lines?

does anyone can suggest reagents and protocols for transient transfection (pcDNA 3.1 plasmid) in meduloblastoma cell lines (daoy, d283, d425, d458 and hd-mb03)? We normally use PEI for transient...

07 July 2019 9,029 2 View

Anyone using BIOCOLOR life science assays?

We recently bought 3 kit to measure collagen and elastin in cells... The manuals are very confusing, badly written with sparse informations... But the worst is to contact costumer/technical...

09 September 2018 3,023 3 View

Does anyone know which mutations the cell lines Huh7, Hep G2, Hep 3b have?

We basically need to know if the cell lines have PIK3CA activation. Nonetheless, is not easy to find informations in ATCC website. Thank you for your help. best

10 October 2016 4,328 4 View

How to remove non-tumour cells from a mouse tumour?

We have a breast cancer mouse model from which we want to extract the tumour, dissociate in single cells and then remove all non-tumour cells to have a pure population of tumour cells to inject in...

10 October 2016 2,260 2 View

Can someone recommended antibodies to perform a co-staining (IHC-P) of CD44/CD24 in paraffin embedded sections (mouse samples)?

Is it possible to do a co-staining in paraffin embedded section with CD44/CD24 antibodies? Mouse samples... thanks

08 August 2016 8,764 2 View

Does anyone added NAD+ (or any precursor) in culture media?

I am working with human cancer cell lines and the goal is to increase NAD+ content in the cells. Nonetheless, I have no idea about the concentration that I should add, if NAD+ (or any precursor)...

03 March 2016 7,592 3 View

Does anyone added citrate and/or pyruvate in culture media?

I am working with human cancer cell lines and the goal is to add sodium citrate and/or sodium pyruvate to the culture media. Nonetheless, I have no idea about the concentration that I should...

02 February 2016 9,530 4 View

Did anyone used an inducible system that express rtTA ubiquitously in transgenic mice?

We are engaged in a metabolic project where we want to express our gene in a whole body and inducible fashion (or at least in important metabolic tissues as muscle, WAT, BAT and liver). Do anyone...

01 January 2015 5,336 4 View